Department of Epidemiology, Tulane School of Public Health and Tropical Medicine, New Orleans, LA, United States.
Department of Epidemiology, Tulane School of Public Health and Tropical Medicine, New Orleans, LA, United States.
Nutr Metab Cardiovasc Dis. 2020 Oct 30;30(11):2077-2084. doi: 10.1016/j.numecd.2020.06.015. Epub 2020 Jun 29.
To examine the associations between history of gestational diabetes mellitus (GDM) and breastfeeding with branched-chain amino acids (BCAA) and their metabolites in later life.
638 women (mean age 48.0 y) who had participated in the Bogalusa Heart Study and substudies of pregnancy history had untargeted, ultrahigh performance liquid chromatography-tandem mass spectroscopy conducted by Metabolon© on serum samples. Metabolites were identified that were BCAA or associated with BCAA metabolic pathways. History of GDM at any pregnancy (self-reported, confirmed with medical records when possible) as well as breastfeeding were examined as predictors of BCAA using linear models, controlling for age, race, BMI, waist circumference, and menopausal status. None of the BCAA differed statistically by history of either GDM or breastfeeding, although absolute levels of each of the BCAA were higher with GDM and lower with breastfeeding. Of the 27 metabolites on the leucine, isoleucine and valine metabolism subpathway, 1-carboxyethylleucine, 1-carboxyethyvaline, and 3-hydroxy-2-ethylpropionate were higher in women with a history of GDM, but lower in women in women with a history of breastfeeding. Similar results were found for alpha-hydroxyisocaproate, 1-carboxyethylisoleucine, and N-acetylleucine.
GDM and breastfeeding are associated in opposite directions with several metabolites on the BCAA metabolic pathway.
研究妊娠期糖尿病（GDM）病史和母乳喂养与支链氨基酸（BCAA）及其在以后生活中的代谢物之间的关联。
638 名（平均年龄 48.0 岁）曾参加过博加卢萨心脏研究（Bogalusa Heart Study）及其妊娠史亚研究的女性，采用 Metabolon©对血清样本进行了非靶向、超高液相色谱-串联质谱分析。鉴定出与 BCAA 代谢途径相关的 BCAA 或代谢物。使用线性模型，通过年龄、种族、BMI、腰围和绝经状态进行控制，将任何妊娠时期的 GDM 病史（自我报告，如有可能通过病历确认）以及母乳喂养作为 BCAA 的预测因子进行检查。尽管 GDM 和母乳喂养对每种 BCAA 的绝对水平都有影响，但它们与 GDM 或母乳喂养的历史无关，BCAA 的统计学差异。在亮氨酸、异亮氨酸和缬氨酸代谢亚途径的 27 种代谢物中，1-羧乙基亮氨酸、1-羧乙基缬氨酸和 3-羟基-2-乙基丙酸在患有 GDM 的女性中较高，但在有母乳喂养史的女性中较低。α-羟基异己酸、1-羧乙基异亮氨酸和 N-乙酰亮氨酸也有类似的结果。
GDM 和母乳喂养与 BCAA 代谢途径上的几种代谢物呈相反方向相关。
