Hirschberg L, Bölske G, Holme T
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden.
Hybridoma. 1989 Apr;8(2):249-57. doi: 10.1089/hyb.1989.8.249.
Intraperitoneal passage in mice and antibiotic treatment were evaluated alone and in combination for elimination of mycoplasma contamination of mouse myeloma cell cultures. Intentional infections were established by inoculating Mycoplasma arginini, M. fermentans, M. hyorhinis and M. orale into cell cultures. Successful elimination of mycoplasmas was achieved with all strains tested by intraperitoneal passage in mice, however, cells infected with M. hyorhinis did not survive the infection long enough to be tested. Clindamycin and lincomycin cured cells infected with M. arginini, M. hyorhinis, M. orale but not M. fermentans. M. fermentans were resistant to all antibiotics tested, but could be partially suppressed by clindamycin long enough to permit curing by in vivo passage. M. arginini was eliminated by all antibiotics tested. In vivo passage and treatment with antibiotics is an efficient combination of methods for mycoplasma elimination from cell cultures and has the advantage of being simple and inexpensive.
单独及联合评估了小鼠腹腔传代和抗生素处理对消除小鼠骨髓瘤细胞培养物支原体污染的效果。通过将精氨酸支原体、发酵支原体、猪鼻支原体和口腔支原体接种到细胞培养物中建立了故意感染。通过在小鼠中进行腹腔传代，所有测试菌株的支原体均成功消除，然而，感染猪鼻支原体的细胞在感染后存活时间不足，无法进行测试。克林霉素和林可霉素可治愈感染精氨酸支原体、猪鼻支原体、口腔支原体的细胞，但不能治愈发酵支原体。发酵支原体对所有测试抗生素均耐药，但可被克林霉素部分抑制足够长的时间，以允许通过体内传代治愈。所有测试抗生素均可消除精氨酸支原体。体内传代和抗生素处理是从细胞培养物中消除支原体的有效方法组合，具有简单且廉价的优点。
