Zhang Yuzhao, Zhao Junhua, Yu Haibo, Li Pan, Liang Wenfeng, Liu Zhu, Lee Gwo-Bin, Liu Lianqing, Li Wen Jung, Wang Zhenning
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.
Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China.
Sci Adv. 2020 Aug 5;6(32):eaba9628. doi: 10.1126/sciadv.aba9628. eCollection 2020 Aug.
Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients' peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an optically induced electrokinetics (OEK) microfluidic method for label-free separation and characterization of patient gastric cancer cells. This method showed high effectiveness and sensitivity. We successfully separated cancer cells from a simulated peritoneal lavage mixture of gastric cancer cell lines and peritoneal lavage cells in a ratio of 1:1000. We further separated gastric cancer cells from six patients' ascites with purity up to 71%. In addition, we measured the cell membrane capacitances, which may be used as a biomarker for gastric cancer cells. Thus, our method can be used to effectively and rapidly detect peritoneal metastasis and to acquire cellular electrical information.
检测腹腔灌洗液和腹水中的游离胃癌细胞在胃癌研究中起着至关重要的作用。然而，由于患者腹腔灌洗液中癌细胞含量较低，传统检测方法缺乏敏感性，无法满足临床需求。在本研究中，我们采用光诱导电动（OEK）微流控方法对患者胃癌细胞进行无标记分离和表征。该方法显示出高效性和敏感性。我们成功地从胃癌细胞系与腹腔灌洗细胞按1:1000比例混合的模拟腹腔灌洗液中分离出癌细胞。我们进一步从6例患者的腹水中分离出胃癌细胞，纯度高达71%。此外，我们测量了细胞膜电容，其可用作胃癌细胞的生物标志物。因此，我们的方法可用于有效、快速地检测腹膜转移并获取细胞电学信息。
