Wang Ao, Liang Shuzhang, Ni Caiding, Jia Yongyi, Wu Kangjie, Niu Wenyan, Huang Shunxiao, Peng Kaiyi, Wang Chutian, Guo Yingjian, Zhao Zhijun, Zhang Lingze, Liu Mingjie, Feng Lin
School of Mechanical Engineering and Automation, Beihang University, Beijing, 100191, China.
Department of Mechanical Engineering, The University of Tokyo, Tokyo, 113-8656, Japan.
Adv Sci (Weinh). 2025 May 5:e2501032. doi: 10.1002/advs.202501032.
Manipulation of single cells or particles is crucial in the biomedical field. However, precisely and rapidly manipulating single cells without damaging them is a significant challenge. In this study, a novel strategy for indirect manipulation of cells and microparticles that can satisfy these requirements via a combination of particle-induced dielectrophoretic forces (PiDEP) and optoelectronic tweezers (OET) is developed. This strategy is based primarily on the principle that particles experiencing the same dielectrophoretic forces tend to repel each other, whereas those experiencing different forces are attracted to each other. During the manipulation, Ag-SiO microparticles controlled by the OET act as intermediaries for manipulating other particles or cells through dielectrophoretic forces. Thus, the manipulation range of the OET can be expanded by two to three times its original size, and the manipulation speed can be significantly increased while maintaining its precision. Furthermore, the results indicate that the proposed method can effectively reduce cell damage to one-third of that caused by traditional OET. This study demonstrates the significant potential of particle-assisted OET for single-cell manipulation and offers an effective strategy for manipulating cells and microparticles.
在生物医学领域，对单个细胞或颗粒进行操控至关重要。然而，在不损伤细胞的情况下精确且快速地操控单个细胞是一项重大挑战。在本研究中，开发了一种新颖的间接操控细胞和微粒的策略，该策略通过结合粒子诱导介电泳力（PiDEP）和光电镊子（OET）来满足这些要求。此策略主要基于这样的原理：受到相同介电泳力的粒子往往相互排斥，而受到不同力的粒子则相互吸引。在操控过程中，由光电镊子控制的银 - 二氧化硅微粒充当通过介电泳力操控其他粒子或细胞的媒介。因此，光电镊子的操控范围可扩大到其原始大小的两到三倍，并且在保持精度的同时操控速度可显著提高。此外，结果表明所提出的方法可将细胞损伤有效降低至传统光电镊子所致损伤的三分之一。本研究证明了粒子辅助光电镊子在单细胞操控方面的巨大潜力，并为操控细胞和微粒提供了一种有效策略。
