State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, PR China.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, PR China.
Anal Chim Acta. 2020 Jun 1;1114:74-84. doi: 10.1016/j.aca.2020.02.041. Epub 2020 Feb 24.
Exosomal biomarkers including tumor-derived exosomes, exosomal surface proteins and exosomal nucleic acids have emerged as one of the most important and general cancer biomarkers in modern biomedical science. These indicators can provide momentous biological information for early diagnosis and treatment of cancer. Recently, numerous studies have been conducted to design biosensors for exosomal biomarkers detection and profiling with high sensitivity and strong applied ability. Among these biosensors, nanomaterial-based optical biosensors are prospective future platforms for rapid and cost-effective detection of exosomal biomarkers. Firstly, we have focused on the progress and advancements in different optical-transducing approaches (Surface-Enhanced Raman Scattering, Surface Plasmon Resonance, Colorimetry, Immunochromatographic assay, Chemiluminescence, Electrochemiluminescence, and fluorescence) for detecting and profiling exosomal biomarkers. Additionally, we have summarized strengths and drawbacks of each strategy. Finally, challenges and future outlooks in developing efficient nanomaterial-based optical biosensor systems for exosomal tumor biomarkers detection have been discussed. The review will exhibit an overview of this field and provide meaningful information for scientific researchers.
外泌体生物标志物包括肿瘤来源的外泌体、外泌体表面蛋白和外泌体核酸，已成为现代生物医学科学中最重要和最普遍的癌症生物标志物之一。这些指标可以为癌症的早期诊断和治疗提供重要的生物学信息。最近，已经有大量的研究致力于设计用于外泌体生物标志物检测和分析的具有高灵敏度和强应用能力的生物传感器。在这些生物传感器中，基于纳米材料的光学生物传感器是用于快速、经济高效地检测外泌体生物标志物的有前途的未来平台。首先，我们重点介绍了用于检测和分析外泌体生物标志物的不同光学转换方法（表面增强拉曼散射、表面等离子体共振、比色法、免疫层析分析、化学发光、电化学发光和荧光）的进展和进步。此外，我们总结了每种策略的优缺点。最后，讨论了开发用于外泌体肿瘤标志物检测的高效纳米材料基光学生物传感器系统的挑战和未来展望。本综述将展示该领域的概述，并为科学研究人员提供有意义的信息。
