Borkowski Andrzej, Kiciński Wojciech, Szala Mateusz, Topolska Justyna, Działak Paweł, Syczewski Marcin D
Faculty of Geology, Geophysics and Environmental Protection, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059 Kraków, Poland.
Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego 2, 00-908 Warsaw, Poland.
Materials (Basel). 2020 Aug 21;13(17):3707. doi: 10.3390/ma13173707.
Carbon-based (nano)materials doped with transition metals, nitrogen and other heteroatoms are considered active heterogeneous catalysts in a wide range of chemical processes. Recently they have been scrutinized as artificial enzymes since they can catalyze proton-coupled electron transfer reactions vital for living organisms. Herein, interactions between Gram-positive and Gram-negative bacteria and either metal-free N and/or S doped or metal containing Fe-N-S co-doped porous carbons are studied. The Fe- and N-co-doped porous carbons (Fe-N-C) exhibit enhanced affinity toward bacteria as they show the highest adsorption capacity. Fe-N-C materials also show the strongest influence on the bacteria viability with visible toxic effect. Both types of bacteria studied reacted to the presence of Fe-doped carbons in a similar manner, showing a decrease in dehydrogenases activity in comparison to controls. The N-coordinated iron-doped carbons (Fe-N-C) may exhibit oxidase/peroxidase-like activity and activate O dissolved in the solution and/or oxygen-containing species released by the bacteria (e.g., HO) to yield highly bactericidal reactive oxygen species. As Fe/N/ and/or S-doped carbon materials efficiently adsorb bacteria exhibiting simultaneously antibacterial properties, they can be applied, inter alia, as microbiological filters with enhanced biofouling resistance.
掺杂过渡金属、氮和其他杂原子的碳基（纳米）材料被认为是广泛化学过程中的活性多相催化剂。最近，它们被作为人工酶进行了详细研究，因为它们可以催化对生物体至关重要的质子耦合电子转移反应。在此，研究了革兰氏阳性菌和革兰氏阴性菌与无金属的氮和/或硫掺杂或含金属的铁-氮-硫共掺杂多孔碳之间的相互作用。铁和氮共掺杂的多孔碳（Fe-N-C）对细菌表现出增强的亲和力，因为它们具有最高的吸附能力。Fe-N-C材料对细菌活力也有最强的影响，具有明显的毒性作用。所研究的两种类型的细菌对掺杂铁的碳的存在反应方式相似，与对照相比，脱氢酶活性降低。氮配位的铁掺杂碳（Fe-N-C）可能表现出类似氧化酶/过氧化物酶的活性，并激活溶解在溶液中的氧和/或细菌释放的含氧物质（如HO），以产生高杀菌活性氧。由于铁/氮和/或硫掺杂的碳材料能有效吸附细菌并同时具有抗菌性能，它们尤其可以用作具有增强抗生物污染能力的微生物过滤器。
