Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry , Central China Normal University , Wuhan , 430079 , People's Republic of China.
School of Mechanical and Materials Engineering , Washington State University , Pullman , Washington 99164 , United States.
Anal Chem. 2019 Sep 17;91(18):11994-11999. doi: 10.1021/acs.analchem.9b02901. Epub 2019 Aug 30.
Recently, in situ detection of hydrogen peroxide (HO) generated from live cells have caused tremendous attention, because it is of great significance in the control of multiple biological processes. Herein, Fe-N-C single-atom nanozymes (Fe-N-C SAzymes) with intrinsic peroxidase-like activity were successfully prepared via high-temperature calcination using FeCl, glucose, and dicyandiamide as precursors. The Fe-N-C SAzymes with FeN as active sites were similar to natural metalloproteases, which can specifically enhance the peroxidase-like activity rather than oxidase-like activity. Accordingly, owing to the excellent catalytic efficiency of the Fe-N-C SAzymes, colorimetric biosensing of HO in vitro was performed via a typical 3,3',5,5'-tetramethylbenzidine induced an allochroic reaction, demonstrating the satisfactory specificity and sensitivity. With regard to the practical application, in situ detection of HO generated from the Hela cells by the Fe-N-C SAzymes was also performed, which can expand the applications of the newborn SAzymes.
最近，对活细胞中产生的过氧化氢（HO）的原位检测引起了极大的关注，因为它在控制多种生物过程中具有重要意义。在此，通过以 FeCl、葡萄糖和双氰胺为前体的高温煅烧，成功制备了具有固有过氧化物酶样活性的 Fe-N-C 单原子纳米酶（Fe-N-C SAzymes）。以 FeN 作为活性位点的 Fe-N-C SAzymes 类似于天然金属蛋白酶，可特异性增强过氧化物酶样活性，而不是氧化酶样活性。因此，由于 Fe-N-C SAzymes 的出色催化效率，通过典型的 3,3',5,5'-四甲基联苯胺诱导的变色反应，在体外对 HO 进行了比色生物传感，表现出令人满意的特异性和灵敏度。关于实际应用，还通过 Fe-N-C SAzymes 对来自 Hela 细胞的 HO 的原位检测进行了研究，这可以扩展新型 SAzymes 的应用。
