Department of Anesthesiology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Department of General Surgery, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150086, China.
Pathol Res Pract. 2020 Sep;216(9):153074. doi: 10.1016/j.prp.2020.153074. Epub 2020 Jun 18.
Emerging evidence has revealed markedly roles for long noncoding RNAs (lncRNAs) in various cancer processes. Prostate cancer associated transcript 6 (PCAT6) is a novel lncRNA which displays vital regulatory functions in multiple cancers. However, the functions of PCAT6 in pancreatic ductal adenocarcinoma (PDAC) remain unclear. Our study confirmed that PCAT6 expression was upregulated in PDAC and the expression of PCAT6 was related to TNM stage, lymph node invasion and overall survival of PDAC patients. PCAT6 might act as an effective tumor biomarker for PDAC patients. Moreover, knockdown of PCAT6 inhibited cell proliferation, migration and invasion of PDAC in vitro. For the mechanism, miR-185-5p expression was decreased and chromobox 2 (CBX2) expression was increased in PDAC, and further PCAT6 could upregulated the expression of oncogene CBX2 by sponging miR-185-5p. The results above suggested that PCAT6/miR-185-5p/CBX2 exerted crucial functions in tumorigenesis and progression of PDAC.
越来越多的证据表明，长非编码 RNA（lncRNA）在多种癌症过程中起着重要作用。前列腺癌相关转录物 6（PCAT6）是一种新型 lncRNA，在多种癌症中具有重要的调节功能。然而，PCAT6 在胰腺导管腺癌（PDAC）中的作用尚不清楚。我们的研究证实，PCAT6 在 PDAC 中表达上调，并且 PCAT6 的表达与 PDAC 患者的 TNM 分期、淋巴结浸润和总生存率有关。PCAT6 可能是 PDAC 患者的有效肿瘤标志物。此外，体外敲低 PCAT6 抑制 PDAC 细胞的增殖、迁移和侵袭。其机制为，PDAC 中 miR-185-5p 的表达降低，染色质盒 2（CBX2）的表达增加，进一步通过海绵吸附 miR-185-5p，PCAT6 上调癌基因 CBX2 的表达。上述结果表明，PCAT6/miR-185-5p/CBX2 在 PDAC 的发生和进展中发挥着重要作用。
