监测 SARS-CoV-2 感染后的抗体反应：4 种自动化免疫分析的诊断效率。
Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays.
机构信息
Department of Clinical Chemistry, Laboratoire Hospitalier Universitaire de Bruxelles (LHUB-ULB), Université Libre de Bruxelles (ULB), Brussels, Belgium.
Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles (LHUB-ULB), Université Libre de Bruxelles (ULB), Brussels, Belgium.
出版信息
Diagn Microbiol Infect Dis. 2020 Nov;98(3):115140. doi: 10.1016/j.diagmicrobio.2020.115140. Epub 2020 Jul 12.
INTRODUCTION
SARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.
MATERIAL AND METHODS
The antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG from Diasorin, the IgG and IgA from Euroimmun, as well as the VIDAS IgG and IgM. Specificity was estimated based on the measurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.
RESULTS
The highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM (78.4%).The Elecsys and the VIDAS IgG/IgM demonstrated a specificity as well as a positive predictive value of 100%.
CONCLUSIONS
The Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results.
简介
SARS-CoV-2 血清转化对于流行病学研究和接触者追踪非常重要。
材料与方法
对 111 例 qRT-PCR 阳性的患者进行了针对 SARS-CoV-2 的抗体反应检测。使用罗氏的 Elecsys、Diasorin 的 Liaison S1/S2 IgG、Euroimmun 的 IgG 和 IgA 以及 VIDAS IgG 和 IgM 评估血清转化。根据在非大流行期间收集的 96 个剩余样本中 SARS-CoV-2 抗体的测量，估计了特异性。
结果
使用 Elecsys（81.1%）、同时检测 IgG/IgM 的 VIDAS（78.4%）和联合检测 IgG/IgA 的 Euroimmun（86.5%），检测血清转化的总体敏感性最高。Elecsys 和 VIDAS IgG/IgM 的特异性和阳性预测值均为 100%。
结论
Elecsys 和 VIDAS 方法结合 IgG/IgM 测量具有高灵敏度且无假阳性结果。
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