Universidad Autónoma de Nuevo León (UANL), Facultad de Ciencias Químicas, Av. Universidad S/N, Cd. Universitaria, C.P. 66455, San Nicolás de los Garza, Nuevo León, Mexico.
Instituto Tecnológico de Sonora (ITSON), Departamento de Ciencias Agronómicas y Veterinarias, 5 de Febrero 818 Sur, C.P. 85000, Ciudad Obregón, Sonora, Mexico.
J Environ Manage. 2020 Dec 1;275:111231. doi: 10.1016/j.jenvman.2020.111231. Epub 2020 Aug 20.
Biocatalytic degradation of recalcitrant pollutants employing ligninolytic enzymes is a promising approach for wastewater treatment. However, enzymes production must be improved to make biodegradation a more cost-effective treatment. In this research, laccase production from Trametes versicolor using lignocellulosic residues (agave bagasse, coconut fibers and wheat bran) as cosubstrates was improved using a central composite face-centered design, and the application of the enzymes-rich culture supernatant was evaluated for blue wastewater biodegradation. Findings revealed that the optimal conditions for laccase production were found at 35 °C and 5 g/L of wheat bran as cosubstrate, reaching about 200 U/mL in 11 days in a batch submerged fermentation. These conditions were scaled up for a submerged fermentation using an airlift reactor, and a maximum enzymatic activity of 1200 U/mL was achieved in 9 days at 30 °C. This enzymes-rich culture supernatant was tested for the degradation of blue wastewater from aircraft in an airlift reactor. Results showed a COD removal efficiency of 43% and an increase of the biodegradability index from 0.64 to 1.36, both results applying an enzymatic activity of supernatant of 300 U/mL. In conclusion, the enzymatic biodegradation becomes a viable strategy for the pretreatment of a real effluent such as the blue wastewater collected in public transportation.
利用木质素降解酶生物催化降解难降解污染物是废水处理的一种很有前途的方法。然而，为了使生物降解成为一种更具成本效益的处理方法，酶的生产必须得到改善。在这项研究中，使用木质纤维素残渣（龙舌兰渣、椰子纤维和麦麸）作为共底物，通过中心复合面心设计提高了彩绒革盖菌产漆酶的能力，并评估了酶丰富的培养上清液在蓝废水生物降解中的应用。结果表明，漆酶生产的最佳条件是在 35°C 和 5 g/L 的麦麸作为共底物下，在分批浸没发酵 11 天内达到约 200 U/mL。在气升式反应器中进行放大浸没发酵，在 30°C 下 9 天内可达到最大酶活 1200 U/mL。该酶丰富的培养上清液在气升式反应器中用于降解飞机蓝废水进行了测试。结果表明，COD 去除效率为 43%，生物降解指数从 0.64 增加到 1.36，这两种结果都采用了 300 U/mL 的上清液酶活。总之，酶法生物降解成为预处理实际废水（如公共交通中收集的蓝废水）的一种可行策略。
