Department of Chemistry, Yonsei University, Seoul 03722, Republic of Korea.
Anal Chem. 2020 Sep 15;92(18):12116-12119. doi: 10.1021/acs.analchem.0c02658. Epub 2020 Aug 25.
Herein we describe the development of the first mitochondrial Cl-selective fluorescent probe, Mito-MQAE, and its applications in biological systems. Fluorescence of Mito-MQAE is insensitive to pH over the physiological pH range and is quenched by Cl with a Stern-Volmer quenching constant of 201 M at pH 7.0. The results of cell studies using Mito-MQAE show that substances with the ability to disrupt mitochondrial membranes cause increases in the mitochondrial Cl concentration.
在此，我们描述了第一个线粒体 Cl 选择性荧光探针 Mito-MQAE 的开发及其在生物系统中的应用。Mito-MQAE 的荧光对生理 pH 范围内的 pH 不敏感，并且在 pH 7.0 时被 Cl 猝灭，Stern-Volmer 猝灭常数为 201 M。使用 Mito-MQAE 的细胞研究结果表明，具有破坏线粒体膜能力的物质会导致线粒体 Cl 浓度增加。
