利用基于荧光视蛋白的主体在生理 pH 值下解锁氯离子感应
Unlocking chloride sensing in the red at physiological pH with a fluorescent rhodopsin-based host.
机构信息
Department of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080, USA.
Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX 75080, USA.
出版信息
Chem Commun (Camb). 2023 Jul 4;59(54):8460-8463. doi: 10.1039/d3cc01786a.
Abstract
Chloride is a vital ion for all forms of life. Protein-based fluorescent biosensors can enable researchers to visualize chloride in cells but remain underdeveloped. Here, we demonstrate how a single point mutation in an engineered microbial rhodopsin results in ChloRED-1-CFP. This membrane-bound host is a far-red emitting, ratiometric sensor that provides a reversible readout of chloride in live bacteria at physiological pH, setting the stage to investigate the roles of chloride in diverse biological contexts.
摘要
氯离子是所有生命形式的重要离子。基于蛋白质的荧光生物传感器可以使研究人员能够在细胞中可视化氯离子，但仍未得到充分发展。在这里，我们展示了在工程化微生物视紫红质中单个点突变如何导致 ChloRED-1-CFP。这种膜结合的宿主是一种远红发射的比率型传感器，可以在生理 pH 值下对活细菌中的氯离子进行可逆读出，为研究氯离子在各种生物背景下的作用奠定了基础。
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3
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Chem Sci. 2022 Oct 6;13(43):12659-12672. doi: 10.1039/d2sc03903f. eCollection 2022 Nov 9.
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Nat Neurosci. 2023 Jan;26(1):64-78. doi: 10.1038/s41593-022-01214-2. Epub 2022 Dec 12.
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Light-driven proton transfers and proton transport by microbial rhodopsins - A biophysical perspective.
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J Neurosci Methods. 2022 Feb 15;368:109455. doi: 10.1016/j.jneumeth.2021.109455. Epub 2021 Dec 22.
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