一种从酿酒酵母中快速制备质粒DNA的方法。
A rapid preparation of plasmid DNA from Saccharomyces cerevisiae.
作者信息
Brozmanová J, Holinová Z
机构信息
Department of Molecular Genetics, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
出版信息
Folia Microbiol (Praha). 1988;33(1):34-7. doi: 10.1007/BF02928011.
A procedure for extraction of plasmid DNA from Saccharomyces cerevisiae is described. The plasmid DNA of interest is extracted together with 2-micron circular DNA naturally occurring in many yeast strains. Spheroplasts are lyzed at alkaline pH which denatures linear but not covalently closed circular (CCC) DNA. The CCC DNA is recovered by ethanol precipitation and can be detected by gel electrophoresis or used for routine bacterial transformation.
本文描述了一种从酿酒酵母中提取质粒DNA的方法。感兴趣的质粒DNA与许多酵母菌株中天然存在的2微米环状DNA一起被提取出来。原生质球在碱性pH值下裂解，使线性DNA变性，但不会使共价闭合环状（CCC）DNA变性。通过乙醇沉淀回收CCC DNA，可通过凝胶电泳检测或将其用于常规细菌转化。
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