在酿酒酵母中，将线性DNA分子在体内连接成环状形式。
In vivo ligation of linear DNA molecules to circular forms in the yeast Saccharomyces cerevisiae.
作者信息
Suzuki K, Imai Y, Yamashita I, Fukui S
出版信息
J Bacteriol. 1983 Aug;155(2):747-54. doi: 10.1128/jb.155.2.747-754.1983.
Abstract
The yeast Saccharomyces cerevisiae was transformed with restriction endonuclease-digested (linear) DNAs containing the replication origin of the yeast 2 microns plasmid and selectable markers with efficiencies of 10(3) to 10(4), 10(3), and 10(2) to 10(3) transformants per microgram of DNA in the cases of transformations with linear DNAs containing the same cohesive ends, flush ends, and non-complementary cohesive ends, respectively. The results of a restriction analysis of the circular plasmids recovered from transformed cells suggested that the linear DNA molecules were ligated to produce circular forms in the recipient protoplasts.
摘要
用含有酵母2μm质粒复制起点和选择标记的经限制性内切酶消化的（线性）DNA转化酿酒酵母，对于分别含有相同粘性末端、平端和非互补粘性末端的线性DNA转化，转化效率分别为每微克DNA产生10³至10⁴、10³和10²至10³个转化子。对从转化细胞中回收的环状质粒进行限制性分析的结果表明，线性DNA分子在受体原生质体中连接形成环状形式。
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