• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

微菌素B17，一种制备最大细胞的新型工具：IncFII微型复制子pMccB17编码的多肽的鉴定。

Microcin B17, a novel tool for preparation of maxicells: identification of polypeptides encoded by the IncFII minireplicon pMccB17.

作者信息

Mayo O, Hernández-Chico C, Moreno F

机构信息

Unidad de Genética Molecular, Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.

出版信息

J Bacteriol. 1988 May;170(5):2414-7. doi: 10.1128/jb.170.5.2414-2417.1988.

DOI:10.1128/jb.170.5.2414-2417.1988
PMID:3283111
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211143/
Abstract

The DNA replication inhibitor peptide microcin B17 is shown to be a useful tool for preparing Escherichia coli maxicells. To illustrate its usefulness, we have identified polypeptides synthesized from pMccB17 and R100 IncFII miniplasmids. After comparing the respective polypeptides and the miniplasmid restriction maps, we concluded that these plasmids share extensive homology in the basic replicon but are different for an adjacent region (parD) that is involved in plasmid stability and maintenance.

摘要

DNA复制抑制剂小菌素B17被证明是制备大肠杆菌最大细胞的有用工具。为了说明其有用性，我们鉴定了从pMccB17和R100 IncFII微型质粒合成的多肽。在比较了各自的多肽和微型质粒限制图谱后，我们得出结论，这些质粒在基本复制子中具有广泛的同源性，但在与质粒稳定性和维持有关的相邻区域（parD）有所不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6ed/211143/b5742ad3a048/jbacter00183-0421-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6ed/211143/b5742ad3a048/jbacter00183-0421-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c6ed/211143/b5742ad3a048/jbacter00183-0421-a.jpg

相似文献

1
Microcin B17, a novel tool for preparation of maxicells: identification of polypeptides encoded by the IncFII minireplicon pMccB17.微菌素B17，一种制备最大细胞的新型工具：IncFII微型复制子pMccB17编码的多肽的鉴定。
J Bacteriol. 1988 May;170(5):2414-7. doi: 10.1128/jb.170.5.2414-2417.1988.
2
Complete nucleotide sequence and comparative genomic analysis of microcin B17 plasmid pMccB17.微菌素 B17 质粒 pMccB17 的完整核苷酸序列和比较基因组分析。
Microbiologyopen. 2024 Apr;13(2):e1402. doi: 10.1002/mbo3.1402.
3
Cloning and mapping of the genetic determinants for microcin B17 production and immunity.微菌素B17产生及免疫的遗传决定因素的克隆与定位
J Bacteriol. 1985 Jul;163(1):275-81. doi: 10.1128/jb.163.1.275-281.1985.
4
Evidence that colicin X is microcin B17.关于大肠杆菌素X是微菌素B17的证据。
J Bacteriol. 1987 Jun;169(6):2899-901. doi: 10.1128/jb.169.6.2899-2901.1987.
5
Molecular characterization of a DNA fragment carrying the basic replicon of pTUC100, the natural plasmid encoding the peptide antibiotic microcin J25 system.携带pTUC100基本复制子的DNA片段的分子特征分析，pTUC100是编码肽抗生素微菌素J25系统的天然质粒。
Plasmid. 2005 May;53(3):258-62. doi: 10.1016/j.plasmid.2004.09.003. Epub 2004 Nov 2.
6
Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12.对大肠杆菌K12中微小菌素B17发挥作用所需的一个基因（sbmA）进行鉴定、定位、克隆及特性分析。
J Gen Microbiol. 1986 Jun;132(6):1685-93. doi: 10.1099/00221287-132-6-1685.
7
Microcin B17 blocks DNA replication and induces the SOS system in Escherichia coli.微菌素B17可阻断大肠杆菌中的DNA复制并诱导SOS系统。
J Gen Microbiol. 1986 Feb;132(2):393-402. doi: 10.1099/00221287-132-2-393.
8
Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid.鉴定质粒R1新稳定性系统ParD的组成部分，该系统靠近此质粒的复制起点。
Mol Gen Genet. 1987 Nov;210(1):101-10. doi: 10.1007/BF00337764.
9
Construction and characterization of mutations at codon 751 of the Escherichia coli gyrB gene that confer resistance to the antimicrobial peptide microcin B17 and alter the activity of DNA gyrase.构建并鉴定大肠杆菌gyrB基因第751位密码子的突变，这些突变赋予对抗菌肽微菌素B17的抗性并改变DNA回旋酶的活性。
J Bacteriol. 2001 Mar;183(6):2137-40. doi: 10.1128/JB.183.6.2137-2140.2001.
10
The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine.DNA复制抑制剂小菌素B17是一种含有60%甘氨酸的43个氨基酸的蛋白质。
Proteins. 1986 Nov;1(3):230-8. doi: 10.1002/prot.340010305.

引用本文的文献

1
Cyclic AMP receptor protein positively controls gyrA transcription and alters DNA topology after nutritional upshift in Escherichia coli.环腺苷酸受体蛋白正向调控大肠杆菌中gyrA的转录，并在营养上调后改变DNA拓扑结构。
J Bacteriol. 1996 Jun;178(11):3331-4. doi: 10.1128/jb.178.11.3331-3334.1996.
2
Structure and organization of plasmid genes required to produce the translation inhibitor microcin C7.产生翻译抑制剂微菌素C7所需的质粒基因的结构与组织
J Bacteriol. 1995 Dec;177(24):7131-40. doi: 10.1128/jb.177.24.7131-7140.1995.
3
appR gene product activates transcription of microcin C7 plasmid genes.

本文引用的文献

1
Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication.质粒R1的复制控制功能可作为复制所需基因表达的抑制剂。
Mol Gen Genet. 1981;184(1):56-61. doi: 10.1007/BF00271195.
2
Genes and sites involved in replication and incompatibility of an R100 plasmid derivative based on nucleotide sequence analysis.基于核苷酸序列分析的R100质粒衍生物复制与不相容性相关的基因和位点
Mol Gen Genet. 1980;179(3):527-37. doi: 10.1007/BF00271742.
3
Analysis of plasmid genome evolution based on nucleotide-sequence comparison of two related plasmids of Escherichia coli.
appR基因产物激活微菌素C7质粒基因的转录。
J Bacteriol. 1989 May;171(5):2906-8. doi: 10.1128/jb.171.5.2906-2908.1989.
4
The peptide antibiotic microcin B17 induces double-strand cleavage of DNA mediated by E. coli DNA gyrase.肽抗生素微菌素B17可诱导由大肠杆菌DNA促旋酶介导的DNA双链断裂。
EMBO J. 1991 Feb;10(2):467-76. doi: 10.1002/j.1460-2075.1991.tb07969.x.
基于大肠杆菌两个相关质粒核苷酸序列比较的质粒基因组进化分析。
Gene. 1982 Mar;17(3):299-310. doi: 10.1016/0378-1119(82)90146-9.
4
The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19.抗性质粒R1drd - 19复制控制区的核苷酸序列。
Mol Gen Genet. 1981;181(1):116-22. doi: 10.1007/BF00339014.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine.DNA复制抑制剂小菌素B17是一种含有60%甘氨酸的43个氨基酸的蛋白质。
Proteins. 1986 Nov;1(3):230-8. doi: 10.1002/prot.340010305.
7
Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid.鉴定质粒R1新稳定性系统ParD的组成部分，该系统靠近此质粒的复制起点。
Mol Gen Genet. 1987 Nov;210(1):101-10. doi: 10.1007/BF00337764.
8
Microcin B17 blocks DNA replication and induces the SOS system in Escherichia coli.微菌素B17可阻断大肠杆菌中的DNA复制并诱导SOS系统。
J Gen Microbiol. 1986 Feb;132(2):393-402. doi: 10.1099/00221287-132-2-393.
9
Characterization of the gene products produced in minicells by pSM1, a derivative of R100.R100的衍生物pSM1在微细胞中产生的基因产物的特性分析。
Mol Gen Genet. 1986 Oct;205(1):56-65. doi: 10.1007/BF02428032.
10
DNA replication of the resistance plasmid R100 and its control.抗性质粒R100的DNA复制及其调控
Adv Biophys. 1986;21:115-33. doi: 10.1016/0065-227x(86)90018-3.