Bravo A, de Torrontegui G, Díaz R
Centro de Investigaciones Biológicas (C.S.I.C.), Velázquez, Madrid, Spain.
Mol Gen Genet. 1987 Nov;210(1):101-10. doi: 10.1007/BF00337764.
We provide evidence that a mutation which derepresses an autoregulated system that is located in the vicinity of the basic replicon of R1, stabilizes the ParA- and ParB- miniplasmid of R1 pKN1562, without increasing its copy number. The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid. Two proteins whose expression is coordinated are components of the system. The sequence of the PstI-EcoRI fragment was obtained. The wild-type ParD system determines in cis a basal but detectable stability.
我们提供的证据表明，一个位于R1基本复制子附近的自动调节系统去阻遏突变，可稳定R1 pKN1562的ParA和ParB微型质粒，而不增加其拷贝数。我们将该系统称为ParD，它定位于与质粒复制起点相邻的1.45 kb PstI-EcoRI片段内。该系统的两个协调表达的蛋白是其组成部分。我们获得了PstI-EcoRI片段的序列。野生型ParD系统顺式决定了一种基础但可检测到的稳定性。
