Aleixo J A, Swaminathan B
Purdue University, Department of Foods & Nutrition, West Lafayette, IN 47907.
J Immunoassay. 1988;9(1):83-95. doi: 10.1080/01971528808053212.
A double antibody sandwich immunoassay (EIA) was developed for the detection of Salmonella. The assay utilizes a beta-galactosidase-murine myeloma monoclonal antibody (M467) conjugate prepared with the heterobifunctional coupling reagent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and uses 4-methyl umbelliferyl beta-D-galactoside as a fluorogenic substrate for the enzyme. The EIA is sensitive and rapid, and compared favorably with the conventional cultural technique in the analysis of 60 feed samples naturally contaminated with Salmonella. Proteins or natural contaminants from the sample did not interfere in the assay.
开发了一种用于检测沙门氏菌的双抗体夹心免疫分析（EIA）方法。该分析方法利用用异双功能偶联剂N-琥珀酰亚胺基3-（2-吡啶基二硫代）丙酸酯（SPDP）制备的β-半乳糖苷酶-鼠骨髓瘤单克隆抗体（M467）结合物，并使用4-甲基伞形基β-D-半乳糖苷作为该酶的荧光底物。该EIA灵敏且快速，在分析60份自然污染沙门氏菌的饲料样品时，与传统培养技术相比具有优势。样品中的蛋白质或天然污染物不会干扰该分析。
