Anderson J M, Hartman P A
Appl Environ Microbiol. 1985 May;49(5):1124-7. doi: 10.1128/aem.49.5.1124-1127.1985.
A direct enzyme immunoassay (EIA) with polyclonal antibodies was developed for detecting salmonellae in foods and feeds. Salmonella cells were attached firmly to the wells of polystyrene microtitration plates with a capture-antibody technique. Spicer-Edwards anti-H immunoglobulin G was bound to protein A-beta-D-galactosidase to serve as the signal; 4-methylumbelliferyl-beta-D-galactoside was used as the substrate. The sensitivity threshold was 10(7) cells per ml. Direct EIA, indirect EIA, and pure-culture techniques were compared by using 48 samples of naturally contaminated foods and feeds. The direct EIA was more sensitive than the indirect EIA or pure-culture technique. Food samples were analyzed within 3 working days, and 32 samples were tested simultaneously in a single 96-well microtitration plate. False-positive or false-negative results did not pose a problem. This direct EIA is sensitive, rapid, and amenable to automation.
开发了一种采用多克隆抗体的直接酶免疫测定法（EIA），用于检测食品和饲料中的沙门氏菌。利用捕获抗体技术将沙门氏菌细胞牢固地附着在聚苯乙烯微量滴定板的孔中。将斯派塞 - 爱德华兹抗 - H免疫球蛋白G与蛋白A-β-D-半乳糖苷酶结合用作信号；4-甲基伞形酮基-β-D-半乳糖苷用作底物。灵敏度阈值为每毫升10(7)个细胞。通过使用48份自然污染的食品和饲料样品，比较了直接EIA、间接EIA和纯培养技术。直接EIA比间接EIA或纯培养技术更灵敏。食品样品在3个工作日内进行分析，并且在单个96孔微量滴定板中可同时检测32个样品。假阳性或假阴性结果不成问题。这种直接EIA灵敏、快速且适合自动化操作。
