Ching C K, Black R, Helliwell T, Savage A, Barr H, Rhodes J M
Department of Medicine, University of Liverpool, London.
J Clin Pathol. 1988 Mar;41(3):324-8. doi: 10.1136/jcp.41.3.324.
Lectin peroxidase histochemical analysis was carried out on pancreatic tissue from patients with pancreatic carcinoma and chronic pancreatitis and from subjects with normal pancreas to find a tumour specific pattern of lectin binding that would aid histological and cytological diagnosis. There were striking differences between the lectin binding characteristics of the different cell types in the normal pancreas. Acinar cells were uniformly positive for binding with wheat germ agglutinin and soy bean agglutinin while islet cells were usually negative for these lectins. Ulex europaeus I lectin however, was found not to be specific for endothelium, showing positivity also for acinar and ductal tissue. Griffonia simplicifolia II lectin was found to be highly specific for ductal epithelium, and because of this was tested in a hamster pancreatic cancer model where it was not specific for ductal epithelium, reflecting differing carbohydrate expression in the hamster pancreas. Pancreatic carcinomas and chronic pancreatitis bound all five lectins without any qualitative distinction from each other or from normal pancreatic tissue, but there was increased intensity of peanut agglutinin binding to secreted mucins in pancreatic carcinoma, which may be of potential use in radiolabelled lectin scanning.
对胰腺癌患者、慢性胰腺炎患者以及胰腺正常者的胰腺组织进行了凝集素过氧化物酶组织化学分析，以寻找有助于组织学和细胞学诊断的肿瘤特异性凝集素结合模式。正常胰腺中不同细胞类型的凝集素结合特征存在显著差异。腺泡细胞与麦胚凝集素和大豆凝集素结合呈均匀阳性，而胰岛细胞对这些凝集素通常呈阴性。然而，发现荆豆凝集素I并非内皮细胞特异性的，腺泡和导管组织也呈阳性。发现西非单叶豆凝集素II对导管上皮具有高度特异性，因此在仓鼠胰腺癌模型中进行了测试，结果显示它对仓鼠胰腺的导管上皮不具有特异性，这反映了仓鼠胰腺中碳水化合物表达的差异。胰腺癌和慢性胰腺炎对所有五种凝集素的结合没有相互之间或与正常胰腺组织的任何定性区别，但胰腺癌中花生凝集素与分泌性粘蛋白的结合强度增加，这可能在放射性标记凝集素扫描中具有潜在用途。
