Oroojan Ali Akbar
Department of Physiology, Faculty of Medicine, Student Research Committee, Dezful University of Medical Sciences, Dezful, Iran.
Int J Endocrinol. 2020 Aug 5;2020:7416529. doi: 10.1155/2020/7416529. eCollection 2020.
Glucose homeostasis is required for control of insulin secretion. Phenolic compounds improved glucose-stimulated insulin secretion (GSIS). Eugenol is a phenolic compound that may increase GSIS. So, it was decided to investigate the effect of eugenol on the insulin secretion and content of pancreatic islets from the male mice. In this experimental study, 3-month-old NMRI mice (20-25 g) were prepared. The pancreatic islets of Langerhans were isolated using the collagenase digestion method and divided into 12 groups: glucose 2.8, 5.6, and 16.7 mM, glucose 2.8 mM + eugenol 50, 100, and 200 M, glucose 5.6 mM + eugenol 50, 100, and 200 M, and glucose 16.7 mM + eugenol 50, 100, and 200 M. The islet's insulin secretion and content were measured after 1 hour and 24 hours incubation at 37°C, respectively, by the ELISA assays method and related commercial kit. Present results showed that all doses of eugenol increased islet's insulin secretion and content in the medium containing glucose concentrations 2.8, 5.6, and 16.7 mM (<0.05). In conclusion, eugenol as a phenolic compound increased insulin secretion and content of pancreatic islets. The moderate dose of this compound enhanced insulin secretion during hypo- and hyperglycemic conditions, as well as a high dose of eugenol, increased insulin content. Finally, present research suggested that the administration of eugenol 100 M was suitable for the early stage of T2DM as well as eugenol 200 M for the advanced stage of this disease.
葡萄糖稳态是控制胰岛素分泌所必需的。酚类化合物可改善葡萄糖刺激的胰岛素分泌（GSIS）。丁香酚是一种可能增加GSIS的酚类化合物。因此，决定研究丁香酚对雄性小鼠胰岛胰岛素分泌和含量的影响。在本实验研究中，制备了3月龄的NMRI小鼠（20 - 25克）。采用胶原酶消化法分离胰岛，并将其分为12组：葡萄糖浓度为2.8、5.6和16.7 mM，葡萄糖2.8 mM + 丁香酚50、100和200 μM，葡萄糖5.6 mM + 丁香酚50、100和200 μM，以及葡萄糖16.7 mM + 丁香酚50、100和200 μM。分别在37°C孵育1小时和24小时后，通过ELISA检测方法和相关商业试剂盒测定胰岛的胰岛素分泌和含量。目前的结果表明，在含有葡萄糖浓度为2.8、5.6和16.7 mM的培养基中，所有剂量的丁香酚均增加了胰岛的胰岛素分泌和含量（P<0.05）。总之，丁香酚作为一种酚类化合物增加了胰岛的胰岛素分泌和含量。该化合物的中等剂量在低血糖和高血糖条件下增强了胰岛素分泌，高剂量的丁香酚增加了胰岛素含量。最后，目前的研究表明，100 μM的丁香酚给药适用于2型糖尿病的早期阶段，而200 μM的丁香酚适用于该疾病的晚期阶段。
