Sheng Yinzhen, Sun Yulong, Zhang Xin, Wan Haifu, Yao Chengjie, Liang Keying, Li Leibin, Liu Bo, Zhong Jianxing, Zhang Ziping, Wang Yilei
Fisheries College, Jimei University, Xiamen, China.
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.
PeerJ. 2020 Aug 3;8:e9655. doi: 10.7717/peerj.9655. eCollection 2020.
Myostatin (MSTN) is a negative regulator of muscle growth, which restrains the proliferation and differentiation of myoblasts. To understand the role of two genes of , the full-length cDNAs of 1131 bp and 1,080 bp were obtained from the ' genomic database, which encodes 376 and 359 amino acids, respectively. The results of qRT-PCR showed that was expressed in the eye, kidney, spleen, skeletal muscle, gill, and brain, and the expression level in the skeletal muscle was extremely significantly higher than in other examined tissues. was expressed in the skin, skeletal muscle, gill, and brain, and had the highest expression in the skeletal muscle, followed by expression in the brain. Meanwhile, in different stages of embryonic development, the expression of started from the gastrula stage. Its expression in the eye-pigment formation stage and hatching stage was significantly higher than that in other stages. The was expressed in all examined embryonic stages with different levels, and the highest expression was detected in the eye-pigment formation stage. These results suggested that and may involve in the development of skeletal muscle, and may be related to the formation of nervous system.
肌肉生长抑制素（MSTN）是肌肉生长的负调节因子，可抑制成肌细胞的增殖和分化。为了解两个基因的作用，从[物种名称]基因组数据库中获得了长度分别为1131 bp和1080 bp的全长cDNA，它们分别编码376和359个氨基酸。qRT-PCR结果显示，[基因名称1]在眼睛、肾脏、脾脏、骨骼肌、鳃和大脑中表达，且在骨骼肌中的表达水平极显著高于其他检测组织。[基因名称2]在皮肤、骨骼肌、鳃和大脑中表达，在骨骼肌中表达最高，其次是在大脑中表达。同时，在胚胎发育的不同阶段，[基因名称1]从原肠胚阶段开始表达。其在眼色素形成阶段和孵化阶段的表达显著高于其他阶段。[基因名称2]在所有检测的胚胎阶段均有不同水平的表达，在眼色素形成阶段检测到最高表达。这些结果表明，[基因名称1]和[基因名称2]可能参与骨骼肌的发育，[基因名称2]可能与神经系统的形成有关。
