Tangsongcharoen Chontida, Jupatanakul Natapong, Promdonkoy Boonhiang, Dimopoulos George, Boonserm Panadda
Institute of Molecular Biosciences, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, Thailand.
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America.
PLoS One. 2017 Apr 13;12(4):e0175473. doi: 10.1371/journal.pone.0175473. eCollection 2017.
Lysinibacillus sphaericus produces the mosquito larvicidal binary toxin consisting of BinA and BinB, which are both required for toxicity against Culex and Anopheles larvae. The molecular mechanisms behind Bin toxin-induced damage remain unexplored. We used whole-genome microarray-based transcriptome analysis to better understand how Culex larvae respond to Bin toxin treatment at the molecular level. Our analyses of Culex quinquefasciatus larvae transcriptome changes at 6, 12, and 18 h after Bin toxin treatment revealed a wide range of transcript signatures, including genes linked to the cytoskeleton, metabolism, immunity, and cellular stress, with a greater number of down-regulated genes than up-regulated genes. Bin toxin appears to mainly repress the expression of genes involved in metabolism, the mitochondrial electron transport chain, and the protein transporter of the outer/inner mitochondrial membrane. The induced genes encode proteins linked to mitochondrial-mediated apoptosis and cellular detoxification including autophagic processes and lysosomal compartments. This study is, to our knowledge, the first microarray analysis of Bin toxin-induced transcriptional responses in Culex larvae, providing a basis for an in-depth understanding of the molecular nature of Bin toxin-induced damage.
球形赖氨酸芽孢杆菌产生由BinA和BinB组成的杀蚊幼虫二元毒素，这两种毒素对于杀灭库蚊和按蚊幼虫都是必需的。Bin毒素诱导损伤背后的分子机制仍未被探索。我们使用基于全基因组微阵列的转录组分析，以更好地了解库蚊幼虫在分子水平上对Bin毒素处理的反应。我们对Bin毒素处理后6小时、12小时和18小时的致倦库蚊幼虫转录组变化进行分析，发现了广泛的转录特征，包括与细胞骨架、代谢、免疫和细胞应激相关的基因，下调基因的数量多于上调基因。Bin毒素似乎主要抑制参与代谢、线粒体电子传递链以及线粒体外膜/内膜蛋白质转运体的基因表达。诱导表达的基因编码与线粒体介导的凋亡和细胞解毒相关的蛋白质，包括自噬过程和溶酶体区室。据我们所知，这项研究是首次对Bin毒素诱导库蚊幼虫转录反应进行的微阵列分析，为深入了解Bin毒素诱导损伤的分子本质提供了基础。
