用离子载体A 23187或孕酮处理后，非洲爪蟾成熟卵母细胞中的游离钙。
Free calcium in full grown Xenopus laevis oocyte following treatment with ionophore A 23187 or progesterone.
作者信息
Bellé R, Ozon R, Stinnakre J
出版信息
Mol Cell Endocrinol. 1977 Jul;8(1):65-72. doi: 10.1016/0303-7207(77)90018-1.
Free intracellular Ca2+ was monitored in isolated Xenopus laevis oocyte during induced maturation using the Ca2+ -sensitive luminescent protein, aequorin. Internal free Ca2+ was not precisely measured but data suggest it was quite low (in the micromolar range). No change in internal free Ca2+ was detected during maturation induced either by progesterone or by p-chloromercuribenzoate. By contrast, the ionophore A 23187 gave an increase in the free Ca2+ level when there was a raised external Ca2+ (10 mM), conditions which also induce oocyte maturation. About 3 h after progesterone or p-chloromercuribenzoate stimulation, the oocyte membrane potential decreased by about 50 mV while the membrane resistance increased transitorily.
在爪蟾卵母细胞诱导成熟过程中，使用对Ca2+敏感的发光蛋白水母发光蛋白监测游离细胞内Ca2+。内部游离Ca2+未精确测量，但数据表明其相当低（在微摩尔范围内）。在孕酮或对氯汞苯甲酸诱导的成熟过程中，未检测到内部游离Ca2+的变化。相比之下，当外部Ca2+升高（10 mM）时，离子载体A 23187使游离Ca2+水平升高，这种条件也会诱导卵母细胞成熟。在孕酮或对氯汞苯甲酸刺激约3小时后，卵母细胞膜电位降低约50 mV，而膜电阻短暂增加。
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