Wasserman W J, Pinto L H, O'Connor C M, Smith L D
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1534-6. doi: 10.1073/pnas.77.3.1534.
Progesterone causes a rapid increase in the intracellular free calcium level in fully grown amphibian oocytes. When albino Xenopus laevis oocytes were microinjected with the Ca-specific photoprotein aequorin, the calcium-induced luminescence from this protein increased at 40-60 sec after the addition of progesterone and returned to the control level within 5-6 min. No further change in the aequorin glow could be detected through the remainder of the maturation period. This transient increase in cytoplasmic free calcium may be involved in controlling the resumption of oocyte maturation.
孕酮会使完全成熟的两栖类卵母细胞内的游离钙水平迅速升高。当用钙特异性光蛋白水母发光蛋白对非洲爪蟾白化卵母细胞进行显微注射后,加入孕酮后40 - 60秒,该蛋白的钙诱导发光增强,并在5 - 6分钟内恢复到对照水平。在整个成熟过程的剩余时间内,未检测到水母发光蛋白发光有进一步变化。细胞质游离钙的这种短暂增加可能参与控制卵母细胞成熟的恢复。