Development and validation of bioanalytical method for the determination of hydrazinocurcumin in rat plasma and organs by HPLC-UV.

Hydrazinocurcumin is a semi-synthetic analogue of curcumin with superior anticancer and anti-angiogenic activities. In the present work a simple and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method for quantitative evaluation of hydrazinocurcumin in plasma and various organs of rats including liver, kidneys, brain, heart, lungs and spleen was developed. Hydrazinocurcumin was separated using octadecylsilane (Inertsil-ODS-3V) column in an isocratic mode using mobile phase consisting of methanol-acetonitrile- water (36:27:37 v/v) with flow rate of 1.0 ml/min. Ultra violet (UV) detection of hydrazinocurcumin and internal standard was carried out in dual-wavelength mode at 332 nm and 380 nm, respectively. The linearity of hydrazinocurcumin was found in the range 0.05-5 µg/ml with a correlation coefficient of r > 0.999. The developed bioanalytical method shown higher inter-day accuracy (98.04-105.94%) and precision (0.89-10.24). The average recoveries of hydrazinocurcumin from rat plasma and various organs were in the range of 96-101.75% and 92.25-99.0%, respectively. The bioanalytical samples shows good stability of hydrazinocurcumin at different storage and handling conditions. In conclusion, this validated HPLC-UV method could be applied effectively for evaluation of hydazinocurcumin for the pharmacokinetic and organ distribution studies.