Acta Chim Slov. 2020 Dec;67(4):1100-1110.
A reliable RP-HPLC analytical method with UV detection at 421 nm was developed and validated for the quantitative determination of curcumin from rat plasma after oral administration of curcumin loaded nanocochleates (CU-NC) to rats. The chromatographic separation was performed on HIQ SIL, C18 (250 mm × 4.6 mm) column using methanol and water (80:20 v/v) as mobile phase, at 1.0 mL/min flow rate. Validation parameters included linearity, accuracy, precision, and limit of quantitation and detection. Good linearity was obtained over the range of 2.5-100 µg/mL (R2 = 0.9979) of curcumin. The developed HPLC method was precise, with <2% relative standard deviation. Accuracy, stability, and robustness studies were also found to be acceptable. Bland-Altman plot showed an acceptable repeatability coefficient. The method was under statistical control, revealed by a control chart. After CU-NC administration, pharmacokinetic parameters i.e. Cmax, AUC0-?, and AUMC0-?, were observed to be 97.69±10.84 µg/mL, 1402.77±9.67 (µg/mL)×h, and 35140.16±14.67 (µg/mL)×h2, respectively. This simple and precise method can be effectively implemented for routine analysis.
建立并验证了一种可靠的反相高效液相色谱法（RP-HPLC），采用紫外检测法（UV），于 421nm 波长处对大鼠口服载姜黄素纳米螺内酯（CU-NC）后大鼠血浆中的姜黄素进行定量测定。采用 HIQ SIL，C18（250mm×4.6mm）柱，以甲醇和水（80:20v/v）为流动相，流速为 1.0mL/min，进行色谱分离。验证参数包括线性、准确度、精密度和定量限及检测限。姜黄素在 2.5-100µg/mL（R2=0.9979）范围内具有良好的线性。所建立的 HPLC 方法精密度良好，相对标准偏差小于 2%。准确度、稳定性和耐用性研究也被认为是可以接受的。Bland-Altman 图显示可接受的重复性系数。该方法处于统计控制之下，这一点通过控制图得以体现。在 CU-NC 给药后，观察到 Cmax、AUC0-?和 AUMC0-?等药代动力学参数分别为 97.69±10.84µg/mL、1402.77±9.67（µg/mL）×h 和 35140.16±14.67（µg/mL）×h2。该方法简单、准确，可有效用于常规分析。
