College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China.
Tissue Cell. 2020 Dec;67:101424. doi: 10.1016/j.tice.2020.101424. Epub 2020 Aug 6.
The carapace from the Chinese soft-shelled turtle (Pelodiscus sinensis) is used as a traditional Chinese medicine. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from turtle carapace were isolated and characterized to screen novel collagen material in this study. Yields of 1.0% and 2.8% were obtained for ASC and PSC which contained glycine as the major amino acid and had high imino acid content. Both collagens had maximum ultraviolet absorption peaks of 220 nm. SDS-PAGE revealed that the structure of both collagens was similar, belonging to type I collagen. Relative viscosities of collagens were decreased as the temperature increased. Collagens showed minimum solubility at pH 8 and maximum solubility at a salt concentration of 3%. The denaturation temperature (Td) of PSC was higher whereas the melting temperature was lower than that of ASC. Both ASC and PSC appeared to be spongy like microstructure with fibrillar pores shown by scanning electron microscopy. The results suggest that collagens isolated from turtle carapace has high thermal stability with potential uses as new substitute for mammalian collagen in medicinal, food or biomaterial fields. However, their biological or pharmacological activities are needed to be further studied.
本研究旨在筛选新型胶原蛋白材料，为此分离并表征了中华鳖（Pelodiscus sinensis）壳中的酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）。分别获得了产率为 1.0%和 2.8%的 ASC 和 PSC，其主要氨基酸为甘氨酸，亚氨基酸含量较高。两种胶原蛋白的最大紫外吸收峰均在 220nm。SDS-PAGE 显示两种胶原蛋白的结构相似，均属于 I 型胶原蛋白。相对粘度随温度升高而降低。胶原蛋白在 pH 8 时溶解度最低，在盐浓度为 3%时溶解度最高。PSC 的变性温度（Td）较高，而熔融温度低于 ASC。扫描电子显微镜显示 ASC 和 PSC 均呈现出海绵状的微观结构，具有纤维状的孔隙。结果表明，从鳖壳中分离得到的胶原蛋白具有较高的热稳定性，有望成为医药、食品或生物材料领域中哺乳动物胶原蛋白的替代品。然而，其生物学或药理学活性尚需进一步研究。
