Chen Wei, Yuan Peng, Yang Ming, Yan Zhiqiang, Kong Siming, Yan Jie, Liu Xixi, Chen Yidong, Qiao Jie, Yan Liying
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.
Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing 100191, China.
Engineering (Beijing). 2020 Oct;6(10):1162-1169. doi: 10.1016/j.eng.2020.07.013. Epub 2020 Aug 17.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread throughout the world, leading to large-scale population infection. Angiotensin-converting enzyme 2 (ACE2) is the receptor of both severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2. However, it is still controversial whether vertical transmission exists. In order to investigate the potential risk of SARS-CoV-2 vertical transmission, we explored and (encoding transmembrane protease serine 2) expression patterns in peri-implantation embryos and the maternal-fetal interface using previously published single-cell transcriptome data. The results showed that day 6 (D6) trophectoderm (TE) cells in peri-implantation embryos, as well as syncytiotrophoblast (STB) at 8 weeks of gestation (STB_8W) and extravillous trophoblast (EVT) cells at 24 weeks of gestation (EVT_24W) in the maternal-fetal interface, strongly co-expressed and , indicating a SARS-CoV-2 infection susceptibility. The positive-expressing cells in the three cell types mentioned above were found to share common characteristics, which were involved in autophagy and immune-related processes. showed no gender bias in post-implantation embryos but showed a significant gender difference in D6_TE, D6 primitive endoderm (PE) cells, and positive-expressing STBs. These findings suggest that there may be different SARS-CoV-2 infection susceptibilities of D6 embryos of different genders and during the gestation of different genders. Our results reveal potential SARS-CoV-2 infection risks during embryo transfer, peri-implantation embryo development, and gestation.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已在全球传播，导致大规模人群感染。血管紧张素转换酶2（ACE2）是严重急性呼吸综合征冠状病毒（SARS-CoV）和SARS-CoV-2的受体。然而，垂直传播是否存在仍存在争议。为了研究SARS-CoV-2垂直传播的潜在风险，我们使用先前发表的单细胞转录组数据，探索了植入前胚胎和母胎界面中跨膜丝氨酸蛋白酶2（TMPRSS2）的表达模式。结果显示，植入前胚胎中的第6天（D6）滋养外胚层（TE）细胞，以及母胎界面中妊娠8周时的合体滋养层（STB_8W）和妊娠24周时的绒毛外滋养层（EVT_24W）细胞，强烈共表达TMPRSS2和ACE2，表明对SARS-CoV-2感染易感。上述三种细胞类型中TMPRSS2阳性表达细胞具有共同特征，这些特征涉及自噬和免疫相关过程。TMPRSS2在植入后胚胎中无性别差异，但在D6_TE、D6原始内胚层（PE）细胞和TMPRSS2阳性表达的STB中存在显著性别差异。这些发现表明，不同性别的D6胚胎以及不同孕期可能存在不同的SARS-CoV-2感染易感性。我们的结果揭示了胚胎移植、植入前胚胎发育和孕期期间潜在的SARS-CoV-2感染风险。
