Kopecný J, Hodrová B
Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Prague.
Lett Appl Microbiol. 1995 May;20(5):312-6. doi: 10.1111/j.1472-765x.1995.tb00453.x.
Pectinolytic enzymes of four rumen fungi have been described. Three fungal species were monocentric Neocallimastix spp. H15, JL3 and OC2, and one isolate was a polycentric strain of Orpinomyces joyonii, A4. They differed in degree of pectin degradation and utilization. Only the strain Neocallimastix sp. H15 and partially Orpinomyces joyonii A4 were able to utilize pectin to a higher extent. The most important pectinolytic activity in all these isolates represented pectin lyase (EC 4.2.2.10) and polygalacturonase (EC 3.2.1.15). Their specific activities were in the range of 100-900 and 10-450 micrograms galacturonic acid h-1 mg protein-1 for pectin lyase and polygalacturonase, respectively. Polygalacturonase, located mainly in the endocellular fraction, was inhibited by calcium ions and had the main pH optimum at pH 6.0. All strains produced pectate lyase (EC 4.2.2.2). None of the strains tested produced pectinesterase (EC 3.1.1.11).
已对四种瘤胃真菌的果胶分解酶进行了描述。三种真菌物种为单中心的新美鞭菌属（Neocallimastix spp.），分别是H15、JL3和OC2，还有一个分离株是多中心的约氏奥尔平菌（Orpinomyces joyonii）A4。它们在果胶降解和利用程度上存在差异。只有新美鞭菌属（Neocallimastix sp.）H15菌株以及部分约氏奥尔平菌（Orpinomyces joyonii）A4能够在更高程度上利用果胶。在所有这些分离株中，最重要的果胶分解活性表现为果胶裂解酶（EC 4.2.2.10）和聚半乳糖醛酸酶（EC 3.2.1.15）。它们的比活性分别为：果胶裂解酶在100 - 900微克半乳糖醛酸·小时⁻¹·毫克蛋白⁻¹范围内，聚半乳糖醛酸酶在10 - 450微克半乳糖醛酸·小时⁻¹·毫克蛋白⁻¹范围内。主要位于细胞内部分的聚半乳糖醛酸酶受到钙离子抑制，其最适pH主要在6.0。所有菌株都产生果胶酸裂解酶（EC 4.2.2.2）。所测试的菌株均未产生果胶酯酶（EC 3.1.1.11）。
