de Moraes Izadora Quintela Souza, do Nascimento Ticiano Gomes, da Silva Antonio Thomás, de Lira Lilian Maria Santos Silva, Parolia Abhishek, Porto Isabel Cristina Celerino de Moraes
Laboratory of Pharmaceutical and Food Analysis, Institute of Pharmaceutical Sciences, Federal University of Alagoas, Campus A. C. Simões, Maceió, Alagoas, Brazil.
Division of Clinical Dentistry, School of Dentistry, International Medical University, Kuala Lumpur, Malaysia.
Restor Dent Endod. 2020 May 22;45(3):e31. doi: 10.5395/rde.2020.45.e31. eCollection 2020 Aug.
Matrix metalloproteinases (MMPs) are enzymes that can degrade collagen in hybrid layer and reduce the longevity of adhesive restorations. As scientific understanding of the MMPs has advanced, useful strategies focusing on preventing these enzymes' actions by MMP inhibitors have quickly developed in many medical fields. However, in restorative dentistry, it is still not well established. This paper is an overview of the strategies to inhibit MMPs that can achieve a long-lasting material-tooth adhesion. Literature search was performed comprehensively using the electronic databases: PubMed, ScienceDirect and Scopus including articles from May 2007 to December 2019 and the main search terms were "matrix metalloproteinases", "collagen", and "dentin" and "hybrid layer". MMPs typical structure consists of several distinct domains. MMP inhibitors can be divided into 2 main groups: synthetic (synthetic-peptides, non-peptide molecules and compounds, tetracyclines, metallic ions, and others) and natural bioactive inhibitors mainly flavonoids. Selective inhibitors of MMPs promise to be the future for specific targeting of preventing dentin proteolysis. The knowledge about MMPs functionality should be considered to synthesize drugs capable to efficiently and selectively block MMPs chemical routes targeting their inactivation in order to overcome the current limitations of the therapeutic use of MMPs inhibitors, ., easy clinical application and long-lasting effect.
基质金属蛋白酶（MMPs）是一类能够降解混合层中胶原蛋白并缩短粘结修复体使用寿命的酶。随着对MMPs科学认识的不断深入，在许多医学领域中，聚焦于通过MMP抑制剂来阻止这些酶发挥作用的有效策略迅速发展起来。然而，在口腔修复学领域，这一策略仍未得到充分确立。本文综述了抑制MMPs的策略，这些策略能够实现材料与牙齿的长期粘结。通过电子数据库全面进行文献检索：PubMed、ScienceDirect和Scopus，检索时间为2007年5月至2019年12月，主要检索词为“基质金属蛋白酶”、“胶原蛋白”、“牙本质”和“混合层”。MMPs的典型结构由几个不同的结构域组成。MMP抑制剂可分为两大类：合成抑制剂（合成肽、非肽分子和化合物、四环素、金属离子等）和天然生物活性抑制剂，主要是黄酮类化合物。MMPs的选择性抑制剂有望成为未来特异性靶向预防牙本质蛋白水解的方向。应考虑MMPs的功能知识，以合成能够有效且选择性地阻断MMPs化学途径从而使其失活的药物，以克服目前MMP抑制剂治疗应用中的局限性，即临床应用简便和效果持久。
