福尔马林固定石蜡包埋及矿化组织中的原位酶谱分析

In Situ Zymography in Formalin-Fixed Paraffin-Embedded and Mineralized Tissues.

作者信息

de Lima Gonçalves Juliana, Magalhães Nilza Letícia, de Queiroz Alexandra Mussolino, Paula-Silva Francisco Wanderley Garcia

机构信息

Developmental Biology and Child Health Group, School of Dentistry of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.

出版信息

Methods Mol Biol. 2025;2918:11-19. doi: 10.1007/978-1-0716-4482-9_2.

DOI:10.1007/978-1-0716-4482-9_2

PMID:40261610

Abstract

Zymography is a method used to detect proteolytic activity in biological samples. There are different ways to perform this technique according to the aim and the samples in the study. In situ zymography is a type of enzymatic analysis that can be applied to histological slides and small tissue samples to identify and localize metalloprotease activity. This methodology can be applied in studies to investigate changes in proteases activity and help to elucidate the pathogenesis of diseases and response to treatment in medical and dental research. In this chapter, the steps to perform in situ zymography in histological slides and mineralized tissue sections are described.

摘要

酶谱法是一种用于检测生物样品中蛋白水解活性的方法。根据研究目的和样品的不同，有不同的方法来实施这项技术。原位酶谱法是一种酶分析类型，可应用于组织学切片和小组织样本，以识别和定位金属蛋白酶活性。该方法可用于研究蛋白酶活性的变化，并有助于阐明医学和牙科研究中疾病的发病机制及对治疗的反应。在本章中，将描述在组织学切片和矿化组织切片中进行原位酶谱法的步骤。