State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Room 7003, N22 Building, Avenide da Universidade, Taipa, Macao 999078, China.
J Agric Food Chem. 2020 Oct 7;68(40):11170-11181. doi: 10.1021/acs.jafc.0c04031. Epub 2020 Sep 24.
()-Oxyphylla A, a natural product isolated from Miquel as a food and medicinal plant, has been reported previously as a novel chiral compound that possesses a potential therapeutic value for Parkinson's disease (PD). A chiral high-performance liquid chromatography-multiple reaction monitoring-mass spectrometry method was developed to separate oxyphylla A enantiomers and to identify the presence of natural ()-oxyphylla A for the first time. Twelve samples of dried fruits were analyzed in which a large variation in the abundance of enantiomers was observed. Moreover, ()-oxyphylla A was less abundant in all tested samples, whereas fruits harvested from Hainan and Guangdong tended to have relatively higher total concentrations of enantiomers. Additionally, enantiomers exhibited comparable neuroprotective effects in the zebrafish model of PD without observed toxicity phenotype. The optimized enantioseparation method will be crucial for the quality control of and research on bioactivities facilitates the development of oxyphylla A as a potential therapeutic for neurodegenerative diseases.
()-Oxyphylla A，一种从药用植物 Miq. 中分离得到的天然产物，此前被报道为一种新型手性化合物，具有治疗帕金森病（PD）的潜在价值。本研究建立了一种手性高效液相色谱-多反应监测-质谱方法，用于分离 (-)-Oxyphylla A 对映体，并首次鉴定出天然 (-)-Oxyphylla A 的存在。对 12 个干果实样本进行了分析，观察到对映体丰度存在较大差异。此外，在所有测试样本中，(-)-Oxyphylla A 的丰度较低，而来自海南和广东的果实的对映体总浓度相对较高。此外，(-)-Oxyphylla A 在 PD 斑马鱼模型中表现出相当的神经保护作用，没有观察到毒性表型。该优化的对映体分离方法对于的质量控制至关重要，同时也为生物活性研究提供了便利，有助于将 Oxyphylla A 开发为治疗神经退行性疾病的潜在药物。
