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[通过溴化乙锭荧光记录十六烷基三甲基溴化铵对大肠杆菌CA189荚膜变体膜作用的动力学分析]

[Kinetic analysis of the action of cetyltrimethylammonium bromide on the membranes of the capsular variant of Escherichia coli CA189 recorded by ethidium bromide fluorescence].

作者信息

Puchkov E O, Aponin Iu M, Irkhin A I, Vaniakin E N

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1988 Jan(1):8-11.

PMID:3284249
Abstract

A mathematical model, describing the kinetics of ethidium bromide probe fluorescence in the suspension of E. coli CA 189 capsular antigen after introduction of cetyltrimethylammonium bromide (a detergent), has been developed. Experimental testing of this mathematical model has confirmed that it describes the kinetics of the probe fluorescence augmentation sufficiently accurately. The new model permits the quantitative characterization of the rate of surfactant diffusion through the bacterial capsule.

摘要

已建立一个数学模型,该模型描述了在引入十六烷基三甲基溴化铵(一种去污剂)后,大肠杆菌CA 189荚膜抗原悬浮液中溴化乙锭探针荧光的动力学。对该数学模型的实验测试证实,它能足够准确地描述探针荧光增强的动力学。新模型允许对表面活性剂通过细菌荚膜的扩散速率进行定量表征。

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