用去污剂使鱼腥藻7120菌株分离的异形胞透化。
Permeabilization of isolated heterocysts of Anabaena sp. strain 7120 with detergent.
作者信息
Privalle L S, Burris R H
出版信息
J Bacteriol. 1983 Aug;155(2):940-2. doi: 10.1128/jb.155.2.940-942.1983.
Abstract
Heterocysts isolated from Anabaena sp. strain 7120 with lysozyme plus sonication were permeabilized with the cationic detergent cetyltrimethylammonium bromide, and they then exhibited comparable acetylene reduction activity in the light and dark with an ATP-regenerating system plus dithionite. The detergent diminished the effect of H2 in enhancing acetylene reduction.
摘要
从鱼腥藻7120菌株中分离出的异形胞，经溶菌酶加超声处理后，用阳离子去污剂十六烷基三甲基溴化铵使其透性化，然后在有ATP再生系统和连二亚硫酸盐存在的情况下，它们在光照和黑暗条件下均表现出相当的乙炔还原活性。该去污剂减弱了氢气对增强乙炔还原的作用。
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