Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
Division of Endocrinology and Metabolism, Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
J Cell Sci. 2020 Sep 15;133(18):jcs247593. doi: 10.1242/jcs.247593.
Emerging evidence indicates that proper mitochondrial dynamics are critical for adipocyte differentiation and functional thermogenic capacity. We found that the mitochondrial fission protein dynamin-related protein 1 (DRP1, also known as DNML1) is highly expressed in brown adipose tissue compared to expression in white adipose tissue, and these expression levels increase during brown adipocyte differentiation. Our results reveal that the inhibition of DRP1 using mdivi-1 mitigates beige adipocyte differentiation and differentiation-associated mitochondrial biogenesis. We found that DRP1 is essential for the induction of the early-phase beige adipogenic transcriptional program. Intriguingly, inhibition of DRP1 is dispensable following the induction of beige adipogenesis and adipogenesis-associated mitochondrial biogenesis. Altogether, we demonstrate that DRP1 in preadipocytes plays an essential role in beige and brown adipogenesis.This article has an associated First Person interview with the first author of the paper.
新出现的证据表明，适当的线粒体动力学对于脂肪细胞分化和功能性产热能力至关重要。我们发现，与白色脂肪组织相比，棕色脂肪组织中线粒体裂变蛋白 dynamin-related protein 1（DRP1，也称为 DNML1）表达水平较高，并且在棕色脂肪细胞分化过程中表达水平增加。我们的结果表明，使用 mdivi-1 抑制 DRP1 可减轻米色脂肪细胞分化和分化相关的线粒体生物发生。我们发现 DRP1 对于诱导早期米色脂肪生成转录程序是必需的。有趣的是，在诱导米色脂肪生成和脂肪生成相关的线粒体生物发生之后，抑制 DRP1 是可有可无的。总的来说，我们证明了前脂肪细胞中的 DRP1 在米色和棕色脂肪生成中起着至关重要的作用。本文有一篇与该论文第一作者的相关第一人称采访。
