Haque N, Bari M S, Ahmed S, Paul S K, Nasreen S A, Ahamed F, Islam A, Roy S, Abedin S, Khan M K, Fariha R H, Debnath P
Dr Nazia Haque, Assistant Professor, Department of Microbiology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:
Mymensingh Med J. 2020 Jul;29(3):589-595.
The coronavirus disease (COVID-19) is highly pathogenic viral infection caused by SARS-CoV-2. Currently, COVID-19 has caused global health concern. WHO has declared COVID-19 as a pandemic disease on March 11, 2020 and characterized by fever, dry cough, fatigue, myalgia and chest pain with pneumonia in severe cases. The virus has spread to at least 213 countries and more than 9093827 confirmed cases and 471490 deaths have been recorded. In the beginning, the world public health authorities tried to eradicate the disease in China through quarantine but are now transitioning to prevention strategies worldwide to delay its spread. There are some newly developed and promising methods for detection of SARS-CoV-2, in order to facilitate the development of novel approaches for early diagnosis. Nucleic acid based tests currently offer the most sensitive and early detection and confirmation for SARS-CoV-2 infection. Among them Real-time reverse transcription polymerase chain reaction (rRT-PCR) is the most popular and the "gold standard" testing method for the detection of SARS-CoV-2. The present study was carried out to detect 2019-Novel Coronavirus (2019-nCoV) by rRT-PCR method at Mymensingh Medical College, Mymensingh, Bangladesh from 1st April, 2020 to 31st May, 2020. A total of 14356 samples were tested from four districts of Mymensingh division namely, Mymensingh, Jamalpur, Sherpur, Netrokona and some parts of Sunamganj for rRT-PCR. Among them 1086 (7.5%) patients were positive for SARS-CoV-2. Out of 1086 positive cases 716(65.9%) were male and 370(34.1%) were female with a Mean±SD age 34.1±12 years. Maximum positivity was found in Mymensingh district followed by Netrokona, Jamalpur, Sherpur and Sunamganj respectively. This is the first base line study for genetic detection of 2019-nCoV in Mymensingh division which may reflect the total scenario of Bangladesh situation. We hope this paper will help the researcher to increase the availability, accuracy, and speed of widespread COVID-19 testing throughout the world in this crisis moment.
冠状病毒病（COVID-19）是由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的高致病性病毒感染。目前，COVID-19已引起全球卫生关注。世界卫生组织于2020年3月11日宣布COVID-19为大流行病，其特征为发热、干咳、乏力、肌痛，严重时伴有胸痛和肺炎。该病毒已传播至至少213个国家，已记录的确诊病例超过9093827例，死亡471490例。起初，世界公共卫生当局试图通过隔离在中国根除该疾病，但现在正转向全球预防策略以延缓其传播。有一些新开发且有前景的检测SARS-CoV-2的方法，以便促进早期诊断新方法的开发。基于核酸的检测目前为SARS-CoV-2感染提供最灵敏和早期的检测与确认。其中，实时逆转录聚合酶链反应（rRT-PCR）是检测SARS-CoV-2最常用且“金标准”的检测方法。本研究于2020年4月1日至2020年5月31日在孟加拉国迈门辛市迈门辛医学院采用rRT-PCR方法检测2019新型冠状病毒（2019-nCoV）。从迈门辛专区的四个区，即迈门辛、贾马尔布尔、舍尔布尔、内特拉科纳以及苏纳甘杰的一些地区共检测了14356份样本进行rRT-PCR检测。其中1086例（7.5%）患者SARS-CoV-2呈阳性。在1086例阳性病例中，716例（65.9%）为男性，370例（34.1%）为女性，平均年龄±标准差为34.1±12岁。迈门辛区的阳性率最高，其次分别是内特拉科纳、贾马尔布尔、舍尔布尔和苏纳甘杰。这是迈门辛专区关于2019-nCoV基因检测的首个基线研究，可能反映孟加拉国的总体情况。我们希望本文能帮助研究人员在这一危机时刻提高全球范围内广泛开展COVID-19检测的可及性、准确性和速度。
