Roy S, Paul S K, Barman T K, Ahmed S, Haque N, Mazid R, Debnath P, Roy S A
Dr Sangjukta Roy, Lecturer, Department of Microbiology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh; E-mail:
Mymensingh Med J. 2020 Jul;29(3):596-600.
There is a new public health problem around the world with the emergence and spread of 2019 novel corona virus (2019-nCoV). The disease "coronavirus disease 2019" (COVID-19) was caused by SARS-CoV-2. As virus isolates are unavailable so the public laboratories are now facing a challenge for detecting the virus because there is growing evidence of the outbreak which is more widespread than initially thought. We aimed here to discuss about the current diagnostic methodology for detecting the SARS-CoV-2 in health laboratories. Here we use the Novel Corona virus (2019-nCoV) Nucleic Acid Diagnostic Kit (PCR-Fluorescence Probing) which is a real time reverse transcription polymerase chain reaction (rRT-PCR) test. A total of 230 samples in the department of microbiology, Mymensingh Medical College from 1st, April 2020 were selected for this study. Among them 20(8.69%) were positive for SARS CoV-2 and remaining were negative. Among the positive samples 55% could amplify both the ORF 1ab and N genes. The single gene ORF 1ab or N was positive in 15% and 30% cases respectively. The Ct values (<38) of ORF 1ab gene indicated by FAM dye was 92.8% and N gene curve indicated by ROX dye was 100%. The presence of IC gene curve with Ct values (<38) indicated by CY5 dye among the positives were 70% and 100% in negatives. The Ct values (38-40) of IC (CY5) among the positives were 15%. The present study demonstrates the enormous response capacity of the study kit for detecting SARS-CoV-2 within the laboratories in Bangladesh.
随着2019新型冠状病毒（2019-nCoV）的出现和传播，全球出现了一个新的公共卫生问题。“2019冠状病毒病”（COVID-19）由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起。由于无法获得病毒分离株，公共实验室目前在检测该病毒方面面临挑战，因为越来越多的证据表明疫情比最初想象的更为广泛。我们的目的是讨论目前在卫生实验室检测SARS-CoV-2的诊断方法。在这里，我们使用新型冠状病毒（2019-nCoV）核酸诊断试剂盒（PCR荧光探针法），这是一种实时逆转录聚合酶链反应（rRT-PCR）检测方法。本研究选取了2020年4月1日起孟加拉国迈门辛医学院微生物学系的230份样本。其中20份（8.69%）SARS-CoV-2呈阳性，其余为阴性。在阳性样本中，55%能够扩增开放阅读框1ab（ORF 1ab）和N基因。单基因ORF 1ab或N分别在15%和30%的病例中呈阳性。由FAM染料指示的ORF 1ab基因的Ct值（<38）为92.8%，由ROX染料指示的N基因曲线为100%。阳性样本中由CY5染料指示的内参基因（IC）曲线Ct值（<38）的比例在阳性样本中为70%，阴性样本中为100%。阳性样本中IC（CY5）的Ct值（38 - 40）为15%。本研究证明了该检测试剂盒在孟加拉国实验室中检测SARS-CoV-2的巨大反应能力。
