School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, China.
Department of Plant Sciences, University of Idaho, Moscow, ID, USA.
J Exp Bot. 2020 Dec 31;71(22):6945-6957. doi: 10.1093/jxb/eraa393.
BSD (mammalian BTF2-like transcription factors, synapse-associated proteins, and DOS2-like proteins) is a conserved domain that exists in a variety of organisms, but its function has not been well studied. Here, we identified a novel BSD domain-containing protein (SlBSD1) in tomato (Solanum lycopersicum). Biochemical and microscopy assays indicated that SlBSD1 is a functional transcription factor that is predominantly localized in the nucleus. Loss-of-function and overexpression analyses suggested that SlBSD1 is a novel regulator of vegetative growth and leaf senescence in tomato. SlBSD1-knockdown (-KD) plants exhibited retarded vegetative growth and precocious leaf senescence, whereas SlBSD1-overexpression (-OX) plants displayed the opposite phenotypes. The negative role of SlBSD1 in leaf senescence was also supported by RNA-seq analysis comparing leaf tissues from SlBSD1-KD and wild-type plants. In addition, contents of soluble solids were altered in fruits in the SlBSD1-KD and SlBSD1-OX plants. Taken together, our data suggest that the novel transcription factor SlBSD1 plays important roles in controlling fruit quality and other physiological processes in tomato, including vegetative growth and leaf senescence.
BSD（哺乳动物 BTF2 样转录因子、突触相关蛋白和 DOS2 样蛋白）是一个保守结构域，存在于多种生物体中，但它的功能尚未得到很好的研究。在这里，我们在番茄（Solanum lycopersicum）中鉴定到一个新的 BSD 结构域蛋白（SlBSD1）。生化和显微镜检测表明 SlBSD1 是一种功能性转录因子，主要定位于细胞核中。功能丧失和过表达分析表明，SlBSD1 是番茄营养生长和叶片衰老的一个新的调控因子。SlBSD1 敲低（-KD）植株表现出营养生长迟缓、叶片衰老提前，而 SlBSD1 过表达（-OX）植株则表现出相反的表型。SlBSD1 在叶片衰老中的负调控作用也得到了 SlBSD1-KD 和野生型叶片组织 RNA-seq 分析的支持。此外，SlBSD1-KD 和 SlBSD1-OX 植株果实中的可溶性固形物含量也发生了改变。综上所述，我们的数据表明，这个新的转录因子 SlBSD1 在控制番茄果实品质和其他生理过程中，包括营养生长和叶片衰老，发挥着重要作用。
