Jang Yoon-Hee, Park Jae-Ryoung, Kim Eun-Gyeong, Kim Kyung-Min
Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Korea.
Crop Breeding Division, Rural Development Administration, National Institute of Crop Science, Wanju 55365, Korea.
Biology (Basel). 2022 Jul 1;11(7):1000. doi: 10.3390/biology11071000.
Photosynthesis is an important factor in determining the yield of rice. In particular, the size and efficiency of the photosynthetic system after the heading has a great impact on the yield. Research related to high-efficiency photosynthesis is essential to meet the growing demands of crops for the growing population. Chlorophyll is a key molecule in photosynthesis, a pigment that acts as an antenna to absorb light energy. Improvement of chlorophyll content characteristics has been emphasized in rice breeding for several decades. It is expected that an increase in chlorophyll content may increase photosynthetic efficiency, and understanding the genetic basis involved is important. In this study, we measured leaf color (CIELAB), chlorophyll content (SPAD), and chlorophyll fluorescence, and quantitative trait loci (QTL) mapping was performed using 120 Cheongcheong/Nagdong double haploid (CNDH) line after the heading date. A major QTL related to chlorophyll content was detected in the RM26981-RM287 region of chromosome 11. was finally selected through screening of genes related to chlorophyll content in the RM26981-RM287 region. The relative expression level of the gene of was highly expressed in cultivars with low chlorophyll content, and is expected to have a similar function to BHLH62 of the genus. is expected to increase photosynthetic efficiency by being involved in the chlorophyll content, and is expected to be utilized as a new genetic resource for breeding high-yield rice.
光合作用是决定水稻产量的一个重要因素。特别是抽穗后光合系统的大小和效率对产量有很大影响。与高效光合作用相关的研究对于满足不断增长的人口对作物的需求至关重要。叶绿素是光合作用中的关键分子，是一种作为天线吸收光能的色素。几十年来，水稻育种一直强调提高叶绿素含量特征。预计叶绿素含量的增加可能会提高光合效率，了解其中涉及的遗传基础很重要。在本研究中，我们测量了叶色（CIELAB）、叶绿素含量（SPAD）和叶绿素荧光，并在抽穗期后使用120个清彻/纳东双单倍体（CNDH）株系进行了数量性状位点（QTL）定位。在第11号染色体的RM26981-RM287区域检测到一个与叶绿素含量相关的主要QTL。通过筛选RM26981-RM287区域中与叶绿素含量相关的基因最终选定了[具体基因名称未给出]。[具体基因名称未给出]基因的相对表达水平在叶绿素含量低的品种中高表达，预计其功能与[具体属名未给出]属的BHLH62相似。[具体基因名称未给出]预计通过参与叶绿素含量来提高光合效率，并有望作为高产水稻育种的新遗传资源加以利用。
