Key Laboratory of Zoonosis, Ministry of Education College of Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, People's Republic of China; College of Food Science and Engneering, Jilin University, Changchun, Jilin, 130062, People's Republic of China.
College of Basic Medical Science, Jilin University, Changchun, Jilin, 130062, People's Republic of China.
Ecotoxicol Environ Saf. 2020 Dec 1;205:111062. doi: 10.1016/j.ecoenv.2020.111062. Epub 2020 Aug 22.
Carbon black (CB) and heavy metals are the main components of Particulate Matter (PM). Although the individual toxicities of CB and heavy metals have been extensively studied, the combined toxicity is much less understood. In this study, we choose the nano carbon black (CBNPs) and Pb to simulate fine particles in the atmosphere and study the combined toxic effect on rat alveolar macrophages. The data showed that CBNPs could adsorb Pb to form CBNPs-Pb complex and displayed an altered physical properties by particle characterization. CBNPs-Pb synergistically induced rat alveolar macrophages apoptosis and blocked autophagy flux compared with CBNPs and Pb individually. Consistent with this, CBNPs-Pb could impair the mitochondrial membrane potential (MMP), activate apoptotic signaling pathways, inhibit lysosomal function.
炭黑（CB）和重金属是颗粒物（PM）的主要成分。虽然 CB 和重金属的单独毒性已经得到广泛研究，但它们的联合毒性却知之甚少。在这项研究中，我们选择纳米炭黑（CBNPs）和 Pb 来模拟大气中的细颗粒物，并研究它们对大鼠肺泡巨噬细胞的联合毒性作用。数据显示，CBNPs 可以吸附 Pb 形成 CBNPs-Pb 复合物，并通过颗粒特性显示出改变的物理性质。与 CBNPs 和 Pb 单独作用相比，CBNPs-Pb 协同诱导大鼠肺泡巨噬细胞凋亡并阻断自噬流。与此一致的是，CBNPs-Pb 可以破坏线粒体膜电位（MMP），激活凋亡信号通路，抑制溶酶体功能。
