Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
J Biosci Bioeng. 2020 Dec;130(6):604-609. doi: 10.1016/j.jbiosc.2020.07.019. Epub 2020 Aug 23.
Fatty acid desaturases, especially Δ12 fatty acid desaturases, are key enzymes for the production of unsaturated fatty acids in oleaginous yeasts. In this study, we identified and characterized a gene encoding Δ12 fatty acid desaturase of Pseudozyma antarctica named PaFAD2. Almost all oleic acid (C18:1) was converted to linoleic acid by the heterologous expression of the PaFAD2 gene in Saccharomyces cerevisiae and Lipomyces starkeyi oleaginous yeast. Notably, PaFad2 converted not only oleic acid to linoleic acid, but also palmitoleic acid (C16:1) to 9,12-hexadecadienoic acid (C16:2). These results indicated that the PaFAD2 gene was very useful for the production of polyunsaturated fatty acids in yeast, including oleaginous yeast.
脂肪酸去饱和酶，特别是 Δ12 脂肪酸去饱和酶，是产油酵母中不饱和脂肪酸生成的关键酶。在本研究中，我们鉴定并表征了一株南极假丝酵母的 Δ12 脂肪酸去饱和酶基因，命名为 PaFAD2。通过将 PaFAD2 基因异源表达于酿酒酵母和产油酵母解脂假丝酵母中，几乎所有的油酸（C18:1）都被转化为亚油酸。值得注意的是，PaFad2 不仅可以将油酸转化为亚油酸，还可以将棕榈油酸（C16:1）转化为 9,12-十六碳二烯酸（C16:2）。这些结果表明，PaFAD2 基因对于酵母，包括产油酵母中多不饱和脂肪酸的生产非常有用。
