Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Department of Applied Life Science, Niigata University of Pharmacy and Applied Life Science, 265-1 Higashijima, Akiha-ku, Niigata, 956-8603, Japan.
Appl Microbiol Biotechnol. 2020 Mar;104(6):2537-2544. doi: 10.1007/s00253-020-10401-9. Epub 2020 Feb 5.
The oleaginous yeast Lipomyces starkeyi is a potential cost-effective source for the production of microbial lipids. Fatty acid elongases have vital roles in the syntheses of long-chain fatty acids. In this study, two genes encoding fatty acid elongases of L. starkeyi, LsELO1, and LsELO2 were identified and characterized. Heterologous expression of these genes in Saccharomyces cerevisiae revealed that LsElo1 is involved in the production of saturated long-chain fatty acids with 24 carbon atoms (C24:0) and that LsElo2 is involved in the conversion of C16 fatty acids to C18 fatty acids. In addition, both LsElo1 and LsElo2 were able to elongate polyunsaturated fatty acids. LsElo1 elongated linoleic acid (C18:2) to eicosadienoic acid (C20:2), and LsElo2 elongated α-linolenic acid (C18:3) to eicosatrienoic acid (C20:3). Overexpression of LsElo2 in L. starkeyi caused a reduction in C16 fatty acids, such as palmitic and palmitoleic acids, and an accumulation of C18 fatty acids such as oleic and linoleic acids. Our findings have the potential to contribute to the remodeling of fatty acid composition and the production of polyunsaturated long-chain fatty acids in oleaginous yeasts.
产油酵母拉斯克氏油脂酵母是生产微生物油脂的有潜力的经济型来源。脂肪酸延长酶在长链脂肪酸的合成中起着至关重要的作用。在这项研究中，鉴定并表征了拉斯克氏油脂酵母的两个编码脂肪酸延长酶的基因，LsELO1 和 LsELO2。这些基因在酿酒酵母中的异源表达表明，LsElo1 参与了 24 个碳原子（C24:0）饱和长链脂肪酸的生成，而 LsElo2 参与了 C16 脂肪酸向 C18 脂肪酸的转化。此外，LsElo1 和 LsElo2 都能够延长多不饱和脂肪酸。LsElo1 将亚油酸（C18:2）延长为二十碳二烯酸（C20:2），而 LsElo2 将α-亚麻酸（C18:3）延长为二十碳三烯酸（C20:3）。LsElo2 在拉斯克氏油脂酵母中的过表达导致 C16 脂肪酸如棕榈酸和棕榈油酸的减少，以及 C18 脂肪酸如油酸和亚油酸的积累。我们的发现有可能有助于改变油脂酵母中脂肪酸组成，并生产多不饱和长链脂肪酸。
