Garcia-Navarro S, Malagon M M, Garcia-Herdugo G, Gracia-Navarro F
Department of Cell Biology, Faculty of Sciences, University of Córdoba, Spain.
Gen Comp Endocrinol. 1988 Feb;69(2):188-96. doi: 10.1016/0016-6480(88)90005-6.
The peroxidase-antiperoxidase (PAP) immunocytochemical technique and rabbit antihuman prolactin (PRL) antiserum were used to localize and identify PRL-producing cells in the pars distalis of Bufo calamita, Hyla meridionalis, Alytes cisternasii, Pelobates cultripes, and Rana perezi tadpoles at different stages of development as well as in 1-year-old post-metamorphic animals. This cell type was located throughout the gland in P. cultripes and R. perezi and in the caudal two-thirds in B. calamita, H. meridionalis, and A. cisternasii in premetamorphic animals. These distribution patterns do not show changes throughout development. Morphometry was used to evaluate the changes observed in pars distalis volume and stereological parameters of PRL immunoreactive cells during development. Pars distalis volume increased during the larval growth period and decreased throughout the metamorphic climax. PRL volume density and cellular area showed different patterns in the different species, although the correlation between these parameters suggests a period of great proliferative rate followed by changes in cellular size. The changes observed in PRL total volume suggest the existence of two phases in amphibian development: (i) a period of PRL storage during pre- and prometamorphosis and (ii) a period of release at the metamorphic climax.
采用过氧化物酶-抗过氧化物酶（PAP）免疫细胞化学技术和兔抗人催乳素（PRL）抗血清，对不同发育阶段的绿蟾蜍、南欧雨蛙、西亚产婆蟾、绿蟾蜍蝌蚪以及1岁变态后的动物远侧部中产生PRL的细胞进行定位和鉴定。在绿蟾蜍蝌蚪和绿蟾蜍中，这种细胞类型分布于整个腺体；在变态前的动物中，在绿蟾蜍、南欧雨蛙和西亚产婆蟾的远侧部后三分之二区域有分布。这些分布模式在整个发育过程中没有变化。采用形态测量法评估发育过程中远侧部体积以及PRL免疫反应性细胞的体视学参数的变化。远侧部体积在幼体生长期增加，在变态高峰期整个减小。PRL体积密度和细胞面积在不同物种中表现出不同模式，尽管这些参数之间的相关性表明存在一个增殖率很高的时期，随后是细胞大小的变化。PRL总体积的变化表明两栖动物发育存在两个阶段：（i）变态前和变态初期PRL储存期；（ii）变态高峰期释放期。
