Margolskee J P
Department of Biochemistry of Biophysics, University of California, San Francisco.
Mol Gen Genet. 1988 Mar;211(3):430-4. doi: 10.1007/BF00425696.
We have used the special properties of the spo13-1 mutation in order to study the regulation of yeast meiosis by the mating type loci. We have found that both the rme1-1 mutation and the sca mutation allow haploid meiosis in spo13-1 strains. Therefore, haploid meiosis is regulated in the same manner as diploid meiosis. Unlike rme1-1, the sca mutation allows meiosis through derepression of the silent mating type cassettes; sca strains can sporulate only because they express both MATa and MAT alpha information. We have found further that sca is an allele of SIR2, one of the genes involved in repression of the silent cassettes. Therefore, the RME1 gene is the only known candidate for a master negative regulator through which the MAT locus controls meiosis.
我们利用spo13-1突变的特殊性质来研究交配型基因座对酵母减数分裂的调控。我们发现rme1-1突变和sca突变都能使spo13-1菌株进行单倍体减数分裂。因此，单倍体减数分裂与二倍体减数分裂的调控方式相同。与rme1-1不同，sca突变通过解除对沉默交配型盒的抑制来允许减数分裂；sca菌株能够形成孢子仅仅是因为它们同时表达了MATa和MATα信息。我们进一步发现sca是SIR2的一个等位基因，SIR2是参与抑制沉默盒的基因之一。因此，RME1基因是已知的唯一可能作为主要负调控因子的候选基因，通过它MAT基因座控制减数分裂。
