恶性疟原虫端粒的序列与结构

Sequence and structure of a Plasmodium falciparum telomere.

作者信息

Vernick K D, McCutchan T F

机构信息

Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.

出版信息

Mol Biochem Parasitol. 1988 Mar;28(2):85-94. doi: 10.1016/0166-6851(88)90055-2.

DOI:10.1016/0166-6851(88)90055-2

PMID:3285206

Abstract

We have isolated a 3 kb cloned DNA fragment which originated from a telomere of the human malaria parasite Plasmodium falciparum. The complete nucleotide sequence of the clone is presented. The clone is composed of several distinct structural regions which vary in their sequence complexity. Using oligonucleotide probes for the different structural regions, we analyzed the genetic conservation of sequence organization near telomeres in various strains of P. falciparum. Our results suggest that rapid sequence variability is generated in the vicinity of chromosome ends.

摘要

我们分离出了一个3 kb的克隆DNA片段，它源自人类疟原虫恶性疟原虫的一个端粒。文中给出了该克隆的完整核苷酸序列。该克隆由几个不同的结构区域组成，其序列复杂性各不相同。我们使用针对不同结构区域的寡核苷酸探针，分析了恶性疟原虫各菌株端粒附近序列组织的遗传保守性。我们的结果表明，在染色体末端附近会产生快速的序列变异性。