Department of Pathology, Yale School of Medicine, New Haven, CT 06520, USA.
Biotechniques. 2020 Dec;69(6):460-468. doi: 10.2144/btn-2020-0095. Epub 2020 Aug 27.
Antibodies play a crucial role in basic research and clinical decision-making. However, there are no standardized algorithms or guidelines to ensure their accuracy and validity. There have been efforts to generate consensus, but, with the exception of clinical labs, antibody validation remains variable in the literature and sometimes in clinical practice. Here we focus on immunohistochemistry, an example of a scientific and clinical tool where validation of antibodies is critical. We describe a protocol that we use to validate antibodies specifically for immunohistochemistry, including some of the pillars of antibody validation from Uhlen 2016, as an example of a rigorous approach to build antibody-based tests for both basic and translational science labs and for the clinic.
抗体在基础研究和临床决策中起着至关重要的作用。然而，目前还没有标准化的算法或指南来确保它们的准确性和有效性。尽管已经做出了一些努力来达成共识，但除了临床实验室之外，抗体验证在文献中仍然存在差异，有时在临床实践中也是如此。在这里，我们专注于免疫组织化学，这是一种科学和临床工具，抗体验证至关重要。我们描述了一个专门用于免疫组织化学的抗体验证方案，包括 2016 年 Uhlen 提出的抗体验证的一些要点，这是一种严格的方法，可用于基础和转化科学实验室以及临床，构建基于抗体的测试。
