Zheng Mei-Li, Du Xiang-Peng, Zhao Lei, Yang Xin-Chun
Heart Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
Beijing Key Laboratory of Hypertension Research, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China.
Chin Med J (Engl). 2020 Nov 5;133(21):2565-2572. doi: 10.1097/CM9.0000000000001056.
Recent studies have reported circular RNA (circRNA) expression profiles in various tissue types; however, circRNA expression profile in human epicardial adipose tissue (EAT) remains undefined. This work aimed to compare circRNA expression patterns in EAT between the heart failure (HF) and non-HF groups.
RNA-sequencing was carried out to compare circRNA expression patterns in EAT specimens from coronary artery disease cases between the HF and non-HF groups. Quantitative real-time polymerase chain reaction was performed for validation. Comparisons of patient characteristics between the two groups were using t test, Mann-Whitney U test, and Chi-squared test.
A total of 141 circRNAs substantially different between the HF and non-HF groups (P < 0.05; fold change >2) were detected, including 56 up-regulated and 85 down-regulated. Among them, hsa_circ_0005565 stood out, for it had the highest fold change and was significantly increased in HF patients in quantitative real-time polymerase chain reaction validation. The top highly expressed EAT circRNAs corresponded to genes involved in cell proliferation and inflammatory response, including GSE1, RHOBTB3, HIPK3, UBXN7, PCMTD1, N4BP2L2, CFLAR, EPB41L2, FCHO2, FNDC3B, and SPECC1. The top enriched Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway were positive regulation of metabolic processes and insulin resistance, respectively.
These data indicate EAT circRNAs may contribute to the pathogenesis of metabolic disorders causing HF.
近期研究报道了环状RNA（circRNA）在多种组织类型中的表达谱；然而，人类心外膜脂肪组织（EAT）中的circRNA表达谱仍不明确。本研究旨在比较心力衰竭（HF）组和非HF组EAT中circRNA的表达模式。
对HF组和非HF组冠状动脉疾病患者的EAT标本进行RNA测序，以比较circRNA的表达模式。采用定量实时聚合酶链反应进行验证。两组患者特征的比较采用t检验、Mann-Whitney U检验和卡方检验。
共检测到141种在HF组和非HF组之间有显著差异的circRNA（P<0.05；倍数变化>2），其中56种上调，85种下调。其中，hsa_circ_0005565尤为突出，因为它的倍数变化最高，并且在定量实时聚合酶链反应验证中，HF患者中显著增加。EAT中高表达的circRNA对应的基因涉及细胞增殖和炎症反应，包括GSE1、RHOBTB3、HIPK3、UBXN7、PCMTD1、N4BP2L2、CFLAR、EPB41L2、FCHO2、FNDC3B和SPECC1。富集程度最高的基因本体术语和京都基因与基因组百科全书途径分别是代谢过程的正调控和胰岛素抵抗。
这些数据表明，EAT中的circRNA可能参与导致HF的代谢紊乱的发病机制。
