Abu-Darwish Mohammad Sanad, Cabral Célia, Ali Zulfigar, Wang Mei, Khan Shabana I, Jacob Melissa R, Jain Surendra K, Tekwani Babu L, Zulfiqar Fazila, Khan Ikhlas A, Taifour Hatem, Salgueiro Lígia, Efferth Thomas
Department of Basic and Applied Sciences, Al-Balqa Applied University, Al-Salt, 19117, Jordan.
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, 38677, USA.
Nat Prod Bioprospect. 2020 Oct;10(5):307-316. doi: 10.1007/s13659-020-00259-9. Epub 2020 Aug 27.
In Jordan, Salvia ceratophylla L. is traditionally used in the treatment of cancer, microbial infections, and urinary disorders. This study aimed: (1) to chemically characterize S. ceratophylla essential oil (EO) from South Jordan, by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS); and (2) to evaluate in vitro the cytotoxic, anti-inflammatory, and antiprotozoal activities of the EO, it's predominant components, and the hexane (A), ethyl acetate (B), methanol (C) and crude-methanol extracts (D). The analysis revealed that the EO has 71 compounds, with linalool (54.8%) as main constituent. Only the hexane extract (A) showed some cytotoxic activity against SK-MEL, KB, BT-549, SK-OV-3, LLC-PK1 and VERO cells lines with IC between 60 and > 100 µg/mL. The EO inhibited NO production (IC 90 µg/mL) and NF-κB activity (IC 38 µg/mL). The extracts A, B, and D inhibited NO production and NF- κB activity with IC between 32 and 150 µg/mL. Linalool considerably inhibited NO production (IC 18 µg/mL). The extracts tested did not exhibit antileishmanial activity. Regarding antitrypanosomal activity, the EO exhibited significant results with IC 2.65 µg/mL. In conclusion, Jordan S. ceratophylla EO represents a rich source of linalool and bears a promising therapeutic potential for further antitrypanosomal drug development.
在约旦，刺叶鼠尾草传统上用于治疗癌症、微生物感染和泌尿系统疾病。本研究旨在：（1）通过气相色谱（GC）和气相色谱-质谱联用（GC-MS）对约旦南部的刺叶鼠尾草精油（EO）进行化学表征；（2）体外评估该精油、其主要成分以及己烷（A）、乙酸乙酯（B）、甲醇（C）和粗甲醇提取物（D）的细胞毒性、抗炎和抗原虫活性。分析表明，该精油含有71种化合物，其中芳樟醇（54.8%）为主要成分。仅己烷提取物（A）对SK-MEL、KB、BT-549、SK-OV-3、LLC-PK1和VERO细胞系显示出一定的细胞毒性活性，IC值在60至>100μg/mL之间。该精油抑制一氧化氮（NO）生成（IC为90μg/mL）和核因子κB（NF-κB）活性（IC为38μg/mL）。提取物A、B和D抑制NO生成及NF-κB活性，IC值在32至150μg/mL之间。芳樟醇显著抑制NO生成（IC为18μg/mL）。所测试的提取物未表现出抗利什曼原虫活性。关于抗锥虫活性，该精油表现出显著效果，IC值为2.65μg/mL。总之，约旦刺叶鼠尾草精油是芳樟醇的丰富来源，在抗锥虫药物进一步开发方面具有广阔的治疗潜力。
