Alves-Silva Jorge M, Cocco Emma, Piras Alessandra, Gonçalves Maria José, Silva Ana, Falconieri Danilo, Porcedda Silvia, Cruz Maria Teresa, Maxia Andrea, Salgueiro Lígia
Institute for Clinical and Biomedical Research, Health Sciences Campus, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Faculty of Pharmacy, Health Sciences Campus, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.
Plants (Basel). 2023 Jan 12;12(2):359. doi: 10.3390/plants12020359.
is widely recognized for its therapeutic potential. However, the biological relevance of some species remains unknown, namely Benth. Therefore, the aim of this study is to unveil the chemical composition and relevant properties to its essential oil (EO). The EO was characterized by GC and GC-MS and its antifungal effect was evaluated according to the CLSI guidelines on dermatophytes and yeasts. The anti-inflammatory potential was assessed on lipopolysaccharide-stimulated macrophages, by assessing the production of nitric oxide (NO) and the effect on the protein levels of two key pro-inflammatory enzymes, iNOS and COX-2 by western blot analysis. Wound healing capacity was determined using the scratch wound healing assay, and the anti-aging potential was assessed by evaluating the senescence marker β-galactosidase. The EO was mainly characterized by γ-curcumene, β-bisabolene, bicyclogermacrene and curzerenone. It is effective in inhibiting the growth of dermatophytes and . The EO significantly decreased iNOS and COX-2 protein levels and concomitantly reduced NO release. Additionally, it demonstrated anti-senescence potential and promoted wound healing. Overall, this study highlights relevant pharmacological properties of the EO of , which should be further explored envisaging the development of sustainable, innovative, and environmentally friendly skin products.
因其治疗潜力而被广泛认可。然而，某些物种的生物学相关性仍然未知，即本特氏菌。因此，本研究的目的是揭示其精油（EO）的化学成分和相关特性。通过气相色谱（GC）和气相色谱 - 质谱联用（GC-MS）对EO进行表征，并根据美国临床和实验室标准协会（CLSI）关于皮肤癣菌和酵母菌的指南评估其抗真菌作用。通过评估一氧化氮（NO）的产生以及通过蛋白质印迹分析评估其对两种关键促炎酶诱导型一氧化氮合酶（iNOS）和环氧化酶 - 2（COX-2）蛋白质水平的影响，在脂多糖刺激的巨噬细胞上评估其抗炎潜力。使用划痕伤口愈合试验确定伤口愈合能力，并通过评估衰老标志物β-半乳糖苷酶来评估其抗衰老潜力。EO的主要特征成分是γ-姜黄烯、β-红没药烯、双环吉马烯和莪术酮。它对抑制皮肤癣菌的生长有效。EO显著降低了iNOS和COX-2的蛋白质水平，并同时减少了NO的释放。此外，它还表现出抗衰老潜力并促进伤口愈合。总体而言，本研究突出了该植物EO的相关药理特性，应进一步探索以开发可持续、创新和环保的皮肤产品。
