[对固醇代谢改变的酵母的维生素D活性评估]
[Evaluation of the vitamin D activity of yeasts with altered sterol metabolism].
作者信息
Mikhaĭlova N P, Valinietse M Iu, Bauman V K, V'iunov K A
出版信息
Prikl Biokhim Mikrobiol. 1988 Jan-Feb;24(1):98-101.
PMID:3285341
Abstract
The sterol content of Saccharomyces strains with altered ergosterol metabolism was studied by UV-spectrophotometry, thin-layer chromatography and chromatographic mass-spectroscopy. A technique for estimation of D-vitamin activity of the yeast strains is proposed. The irradiated biomass of the strains accumulated ergosta-5,7-dien-3 beta-ol and also cholesta-5,7,24-trien-3 beta-ol and cholesta-5,7,22,24-tetraen-3 beta-ol is characterized by high antirachitic activity.
摘要
通过紫外分光光度法、薄层色谱法和色谱质谱法研究了麦角固醇代谢改变的酿酒酵母菌株的甾醇含量。提出了一种评估酵母菌株D-维生素活性的技术。这些菌株经辐照后的生物质积累了麦角甾-5,7-二烯-3β-醇，以及胆甾-5,7,24-三烯-3β-醇和胆甾-5,7,22,24-四烯-3β-醇，其特点是具有高抗佝偻病活性。
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